HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别

李洋; 王慧; 吕磊; 陈俊; 周瑾; 黄玉凤; 周燕妮; 张国庆

doi:10.13422/j.cnki.syfjx.2015130070

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HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别

化学与分析 | 更新时间：2024-01-04

- HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别
- Rapid Identification of Chemical Composition in Ganlibao Capsules by HPLC-TOF/MS
- 中国实验方剂学杂志 2015年21卷第13期页码：70-73
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015130070
  中图分类号： O657.63;R286.0
- 纸质出版日期：2015
- 稿件说明：
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李洋, 王慧, 吕磊, 等. HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别[J]. 中国实验方剂学杂志, 2015,21(13):70-73.

LI Yang, WANG Hui, LYU Lei, et al. Rapid Identification of Chemical Composition in Ganlibao Capsules by HPLC-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 70-73.
李洋, 王慧, 吕磊, 等. HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别[J]. 中国实验方剂学杂志, 2015,21(13):70-73. DOI： 10.13422/j.cnki.syfjx.2015130070.

LI Yang, WANG Hui, LYU Lei, et al. Rapid Identification of Chemical Composition in Ganlibao Capsules by HPLC-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 70-73. DOI： 10.13422/j.cnki.syfjx.2015130070.

摘要

目的: 运用高效液相色谱-高分辨飞行时间质谱(HPLC-TOF/MS)联用技术快速鉴别肝力保胶囊中化学成分。方法: 色谱分离采用SHISEIDO MG C18色谱柱(3.0 mm×100 mm

3 μm);流动相0.1%甲酸水(A)-乙腈(B)

梯度洗脱(0~10 min

5%~20%B;10~30 min

20%~70%B)

柱温25 ℃

流速0.6 mL·min-1

柱后分流比2:1。质谱定性采用飞行时间质谱

电喷雾离子源(ESI)

正负离子模式扫描(m/z 100~1 000)。结果: 一次性鉴别出肝力保胶囊中30种化学成分。结论: 该研究比较全面地阐明了肝力保胶囊的化学组成

为该复方的药效学物质基础研究和质量控制奠定了基础。

Abstract

Objective: To identify chemical composition in Ganlibao capsules by high-performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS) method. Method: The separation was performed on SHISEIDO MG C18 reverse phase column(3.0 mm×100 mm

3 μm). The mobile phase consisted of water containing 0.1% formic acid (A)-acetonitrile (B) was used for gradient eluting (0-10 min

5%-20%B;10-30 min

20%-70%B). Temperature of column was 25 ℃

the flow rate was 0.6 mL·min-1 and post-column split ratio was 2: 1.Time-of-flight mass spectrometer (TOF/MS) and electrospray ion source (ESI) were applied for qualitative analysis under positive and negative ion mode

and mass scan range was m/z 100-1 000. Result: The 30 components of Ganlibao capsules were identified by HPLC-TOF/MS. Conclusion: This method is simple and rapid for elucidating the constituents of Ganlibao capsules

which provides the basis for the research of active composition and quality control for this prescription.

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