HPLC含量测定和DNA分子标记技术对不同产地金银花的鉴定

宋雯舒; 张晨; 甘亮; 黎新银; 严群超

doi:10.13422/j.cnki.syfjx.2015170059

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HPLC含量测定和DNA分子标记技术对不同产地金银花的鉴定

化学与分析 | 更新时间：2024-01-04

- HPLC含量测定和DNA分子标记技术对不同产地金银花的鉴定
- Identification of Lonicerae Japonicae Flos from Different Areas by HPLC Assay and DNA Molecular Markers
- 中国实验方剂学杂志 2015年21卷第17期页码：59-62
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- DOI：10.13422/j.cnki.syfjx.2015170059
  中图分类号： R282.5
- 纸质出版日期：2015
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宋雯舒, 张晨, 甘亮, 等. HPLC含量测定和DNA分子标记技术对不同产地金银花的鉴定[J]. 中国实验方剂学杂志, 2015,21(17):59-62.

SONG Wen-shu, ZHANG Chen, GAN Liang, et al. Identification of Lonicerae Japonicae Flos from Different Areas by HPLC Assay and DNA Molecular Markers[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 59-62.
宋雯舒, 张晨, 甘亮, 等. HPLC含量测定和DNA分子标记技术对不同产地金银花的鉴定[J]. 中国实验方剂学杂志, 2015,21(17):59-62. DOI： 10.13422/j.cnki.syfjx.2015170059.

SONG Wen-shu, ZHANG Chen, GAN Liang, et al. Identification of Lonicerae Japonicae Flos from Different Areas by HPLC Assay and DNA Molecular Markers[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(17): 59-62. DOI： 10.13422/j.cnki.syfjx.2015170059.

摘要

目的: 通过指标成分含量测定及DNA分子标记技术对不同产地金银花样品进行鉴定

探讨DNA分子鉴定技术在中草药鉴别领域的应用前景。方法: 以绿原酸、木犀草苷、灰毡毛忍冬皂苷乙为指标成分

利用HPLC测定指标成分含量。提取金银花、山银花对照药材及河北和山东产地金银花样品DNA

检测DNA浓度和纯度

采用基于聚合酶链式反应(PCR)方法的DNA分子标记技术鉴别样品。结果: 河北和山东产地金银花中绿原酸质量分数3.1%~3.3%

河北、山东产地金银花中木犀草苷质量分数分别为0.049%~0.050%

0.069%~0.078%

2个产地样品中均未检出灰毡毛忍冬皂苷乙成分。采用基于PCR方法鉴定出河北和山东产地各3批样品均为金银花。结论: DNA分子鉴定技术作为含量测定常规方法的补充

具有准确可靠、重复性高等优点

可用于金银花药材的鉴定。

Abstract

Objective: To identify Lonicerae Japonicae Flos from different areas by determination of index ingredients and DNA molecular markers. Method: High performance liquid chromatography method was used for determination of chlorogenic acid

galuteolin and macranthoidin B.Genomic DNA was extracted from control medicinal material of Lonicerae Japonicae Flos and Lonicerae Flos

as well as samples of Lonicerae Japonicae Flos from Hebei and Shandong

samples were identified by allele-specific diagnostic polymerase chain reaction (PCR) based on sequences of internal transcribed spacer. Result: The content of chlorogenic acid in samples from Heibei and Shandong was 3.1%-3.3%.The content of galuteolin in samples from Hebei and Shandong were 0.049%-0.050% and 0.069%-0.078%

respectively.Macranthoidin B was not detected in samples from both areas.Samples from both areas were identified as Lonicerae Japonicae Flos by diagnostic PCR. Conclusion: As a supplement to conventional methods

for identification of Lonicerae Japonicae Flos

DNA molecular technology has a great advantage in accuracy and good reliability.

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