Objective: To investigate the effective hypoglycemic part in different extracts from Leontopodium leontopodioides and its dosage by determining blood glucose in hyperglycemia mice. Method: L. leontopodioides were extracted by 70% ethanol and the extracts were separated by D101 macroporous resin and then eluted successively with 50%

70%

95% of ethanol to obtain 50%

70%

95% of ethanol extracts. Hyperglycemia mice were induced by streptozotocin (STZ) and then perfused respectively with metformin hydrochloride (300 mg· kg-1)

ethanol extracts of 50% (100 mg· kg-1)

ethanol extracts of 70% (100 mg· kg-1) and ethanol extracts of 95% (100 mg· kg-1) after 7 days. The model and blank groups were perfused with the same amount of distilled water. The fasting blood glucose of hyperglycemia mice were measured after 10 days. The screened effective hypoglycemic parts were divided into high (400 mg· kg-1)

medium (200 mg· kg-1) and low (100 mg· kg-1) dose and used to treat hyperglycemia mice;10 days later the fasting blood glucose was measured. Result: The model group showed a significantly higher blood glucose level than the blank group. Compared with the model group

different extracts of L. leontopodioides can reduce hyperglycemia mice blood glucose

the ethanol extracts of 50% had an obvious effect

and the medium dose was the best. Conclusion: The effective hypoglycemic part in L. leontopodioides was 50% ethanol extracts.