鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较

李小莹; 周龙颖珍; 赖丽嫦; 陈丰连

doi:10.13422/j.cnki.syfjx.2015200072

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鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较

化学与分析 | 更新时间：2024-01-04

- 鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较
- Comparison of HPLC Fingerprint of Flavonoid in Dry and Fresh Spatholobi Caulis
- 中国实验方剂学杂志 2015年21卷第20期页码：72-75
- 作者机构：
- 作者简介：
- 基金信息：
  
  广州中医药大学薪火计划项目(A1-AFD015142Z13);广东省科技计划项目(2012B091202024,2012B040500032)
- DOI：10.13422/j.cnki.syfjx.2015200072
  中图分类号： R284.1
- 纸质出版日期：2015
- 稿件说明：
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李小莹, 周龙颖珍, 赖丽嫦, 等. 鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较[J]. 中国实验方剂学杂志, 2015,21(20):72-75.

LI Xiao-ying, ZHOU Long-ying-zhen, LAI Li-chang, et al. Comparison of HPLC Fingerprint of Flavonoid in Dry and Fresh Spatholobi Caulis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 72-75.
李小莹, 周龙颖珍, 赖丽嫦, 等. 鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较[J]. 中国实验方剂学杂志, 2015,21(20):72-75. DOI： 10.13422/j.cnki.syfjx.2015200072.

LI Xiao-ying, ZHOU Long-ying-zhen, LAI Li-chang, et al. Comparison of HPLC Fingerprint of Flavonoid in Dry and Fresh Spatholobi Caulis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(20): 72-75. DOI： 10.13422/j.cnki.syfjx.2015200072.

摘要

目的:建立鸡血藤黄酮部位的HPLC指纹图谱

通过比较同一产地鸡血藤干、鲜品药材的指纹图谱

考察两者化学成分种类和含量上的差异。方法:采用高效液相色谱法

Diamonsil® C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.2%磷酸溶液为流动相梯度洗脱

流速1.0 mL·min-1

检测波长280 nm

检测时间80 min。结果:分别建立了干、鲜鸡血藤黄酮部位的HPLC指纹图谱共有模式

标定了11个共有指纹峰

并指认了4个共有峰

发现干、鲜品在成分和含量上均有差异。结论:该方法简便、准确

具有良好的重复性

可为鸡血藤化学成分深入研究及其加工利用提供科学依据。

Abstract

Objective: To establish the HPLC fingerprint of flavonoid in Spatholobi Caulis

and observe the difference in chemical compositions and content between dry and fresh Spatholobi Caulis from the same origin by comparing the fingerprint of them. Method: HPLC method was adopted. The determination was performed on Diamonsil® C18 column(4.6 mm×250 mm

5 μm) with acetonitrile -0.2% phosphoric acid water solution as eluent gradient at the flow rate of 1.0 mL·min-1. The detection wavelength was 280 nm. The detection time was 80 min. Result: The common mode of HPLC fingerprint of flavonoid in dry and fresh Spatholobi Caulis was set up respectively. There were 11 common peaks in the fingerprint of dry and fresh herbs. Four of them were recognized. Conclusion: The method is simple

accurate and has a good repeatability. It can provide scientific basis for processing and utilization of Spatholobi Caulis.

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