Objective: To observe the antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice and their influence on angiogenesis markers (CD34) and vascular endothelial growth factor (VEGF) expression in sarcoma tissues of tumor-bearing mice. Method: S180 tumor-bearing mice were divided into model group

Xihuang pills group (2.2 g·kg-1·d-1)

Xihuang dropping pills low dose

middle dose and high dose groups (1.5

3

6 g·kg-1·d-1) and cytoxan group (CTX

0.02 g·kg-1). Xihuang pills group

Xihuang dropping pills low dose

middle dose and high dose groups were administered ig

and CTX group was administered ip

1 time/2 days for continuous 10 days. The antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice were observed

and the morphology changes of tumor cells were observed by HE staining. The influence of Xihuang dropping pills on CD34 and VEGF expression was observed by immunohistochemical method. Result: Compared with the model group

the body weight in tumor-bearing mice in Xihuang dropping pills high dose group was significantly increased. The anti-tumor rate in Xihuang pills group

Xihuang dropping pills low

middle

high groups and CTX group was 26.28%

21.47%

26.92%

30.76% and 35.85% respectively. CD34 protein expression in CTX group and Xihuang dropping pills high dose group was significantly lower than that in model group (P<0.05

P<0.01). VEGF protein expression in Xihuang dropping pills middle

high dose group and CTX group was significantly lower than that in model group (P<0.05). There was significant difference in proteins expression between Xihuang dropping pills middle dose group and Xihuang pills group. Conclusion: Xihuang dropping pills have the anti-tumor effect in S180 tumor-bearing mice

and the mechanism may be associated with down-regulating expression of CD34 and VEGF protein in tumor tissues.