Objective: To observe the hemostatic effect and hemostatic mechanism of Rubiae Radix et Rhizoma in treating dysfunctional metrorrhagia. Method: Dysfunctional metrorrhagia models were established by drug abortion in early pregnancy and removal of the ovaries. The rats were divided into normal group

model group

Rubiae Radix et Rhizoma low dose group (1.35 g·kg-1)

middle dose group (2.7 g·kg-1)

high dose group (5.4 g·kg-1) and Gongxuening group (positive drug

0.07 g·kg-1). After treatment for 14 days

the rats were killed to observe the effect on the amount of bleeding in dysfunctional metrorrhagia rats by UV

detect the level of thromboxane B2 (TXB2) and 6-keto-PGF1α contents in blood of rats by enzyme-linked immunosorbent assays

and detect the effect on microvessel density (MVD) expressions of the endometrium in ovariectomized rats by immunohistochemistry. Result: Compared with normal group

the amount of bleeding in model group rats was significantly increased

TXB2 level significantly reduced

6-keto-PGF1α level significantly increased

and endometrial MVD protein expression in ovariectomized rats was significantly reduced (P<0.01). The each dose group of Rubiae Radix et Rhizoma could significantly reduce the amount of bleeding in dysfunctional metrorrhagia rats

significantly increase TXB2 level and significantly reduce 6-keto-PGF1α level (P<0.05). High dose group and middle dose group of Rubiae Radix et Rhizoma could significantly increase endometrial microvascular density level in ovariectomized rats (P<0.05). Conclusion: Rubiae Radix et Rhizoma has a good hemostatic effect

and promotes endometrial repair and blood vessel growth in ovariectomized rats

thus to treat dysfunctional metrorrhagia.