Objective: To observe the estrogen-like effect of the combined administration of Veratri Nigri Radix and Salviae Miltiorrhizae Radix et Rhizoma(SMRR) using immature mice model

so as to provide a scientific basis for eighteen incompatible medicaments Veratri Nigri Radix and SMRR according to theories of traditional Chinese medicine. Method: Totally 10021-day-old healthy Kunming female mice were randomly divided into 10 groups:normal

estradiol valerate (E2

0.1 g·kg-1)

E2 plus estrogen receptor antagonist (ICI

0.005 g·kg-1)

E2 plus Veratri Nigri Radix (VN

0.045 g·kg-1)

SMRR (SM

1.6

3.2 g·kg-1)

SM plus ICI

SM plus VN for 7 days respectively

with 10 mice in each group. The estrogen-like effect was detected by observing uterus index

E2 in serum

LH and FSH levels and histomorphology changes in uterus and vagina. Result: Compared with normal group

SM groups showed significant increase in the uterus index (P<0.01

P<0.05) and decrease in the level of E2 (P<0.01)

the level of LH (P<0.05) and FSH (P<0.01

P<0.05)

and the growth and development of uterus and vagina in immature mice

with a similar effect to E2 (P<0.01). In SM plus VN group

uterus index decreased(P<0.05

P<0.01)

level of E2 notably decreased (P<0.01

P<0.05)

level of LH (P<0.01) and FSH (P<0.05

P<0.01) increased

and the growth and development of uterus and vagina decreased

with a similar trend to the SM plus ICI group. Meanwhile

in E2 plus VN group

uterus index decreased(P<0.01)

level of E2 notably decreased (P<0.01)

level of LH and FSH increased (P<0.01)

and the growth and development of uterus and vagina decreased

with a similar effect to SM plus ICI group. Conclusion: These findings rprove that SMRR exerts estrogenic effect

VN has the anti-estrogen effect and can antagonize the estrogenic efficacy of SMRR.