Objective: To investigate abnormal changes of plasma endogenous metabolic in rats with W256 sarcocarcinoma and regulation of Xiaoai Jiedu prescription (XJP) on metabolic disorder of transplanted tumor rats.To explore mechanisms of XJP on anti-tumor based on metabonomics. Method: W256-transplanted tumor rats model were established and were divided into the control group

the model group and XJR treatment groups.Plasma smaples were collected on the 1st

6th and 11th day after administration.Endogenous metabolites of all groups were determined by GC-MS.Partial least squares-discriminant analysis (PLS-DA) was applied to analyze multivariate data and established model

metabolite identification was adopted database

t test was used in significant statistical analysis and metabolic pathways. Result: Compared with the control group

rats in the model group on the 11th day

30 endogenous metabolites significantly changed

10 endogenous metabolites got differently restoration to the normal group after treated XJR. Conclusion: Thirty endogenous metabolites may be potential metabolic biomarkers.Abnormal metabolite levels in plasma can be partly recovered by XJR

these 10 endogenous metabolites may be biomarker of XJP working.And potential antitumor effects of XJR may be contributed to regulation of related metabolic pathways.