Objective: To determine L-quebrachitol in nature rubber latex serum by establishing high-performance liquid chromatography-diode array detector(HPLC-DAD). Method: Fresh latex was coagulated by 18% acetic acid and unglued by filtration. 0.6% Ca(OH)2 filtration liquid was added and heated to boiling status for removing protein

then the mixture was filtered. Salt and lipid content in the filtrate was removed by anion and cation resin exchange

and the residue achieved adsorption through macroporous adsorption resins

then desorbed by 60% ethanol

and the raw quebrachitol was obtained by Vacuum condensation crystallization. The product was dissolved in ultrapure water with metered volume. L-quebrachitol was separated by Inertsil ODS-SP C18 column with acetonitrile-water(10:90) as mobile phase at a flow rate of 1.0 mL·min-1 and column temperature of 30℃. Result: A good linear relationship was obtained in the range of 0.04-4.0 g·L-1 for L-quebrachitol

with a linear correlation coefficient(R) of 0.9979.The RSD was 1.1%

and the average recovery rate was 96.69%. Conclusion: This method is simple with high sensitivity and precision

suitable for determination of L-quebrachitol in nature rubber latex serum.