PENG Liang, LI Yi-guang, CHEN Jie, et al. Rapid Analysis Method of HPLC Fingerprint Spectrum of Flavonoids in [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 53-56.
PENG Liang, LI Yi-guang, CHEN Jie, et al. Rapid Analysis Method of HPLC Fingerprint Spectrum of Flavonoids in [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 53-56. DOI: 10.13422/j.cnki.syfjx.2016010053.
Objective: To establish the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum. Method: HPLC was performed on ZORBAX SB-C18 column(4.6 mm×150 mm
5μm)
eluting in a gradient mode with the mobile phase of acetonitrile(A)-0.2%H3PO4 as(B) at a flow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 360 nm. Injection volume was 10μL. Result: Eleven common characteristic peaks were identified as fingerprint peaks
the methodological study was consistent with the technical requirements. Conclusion: The established method is stable
reliable and highly accurate
and can provide reference for quality control of Gynostemma pentaphyllum.