Objective: To conduct a qualitative analysis for the quality of Shengxuebao mixture by HPLC

in order to achieve the quality control of the overall accuracy of Shengxuebao mixture

and establish a multi-component fingerprint chromatogram of Shenxuebao mixture by HPLC. Method: Thermo Syncronis-C18 column(4.6 mm×150 mm

5μm) was eluted with the mobile phase of acetonitrile(A) and 0.1% phosphoric acid(B) in a gradient mode(0-15 min

2%A;15-30 min

2%-15%A;30-50 min

15%-25%A;50-60 min

25%A). Detection wavelength was set at 230 nm;column temperature was 30℃;the flow rate was 0.8-1.0 mL·min-1 and analytic time was 60 min. Injection volume was 5μL. Result: Under the same chromatogram condition

paeoniflorin and other condittuents were determined

and 14 peaks were identified on the HPLC fingerprint. The methodological results were satisfactory. The similarity of the 10 batches of Shenxuebao mixture was above 0.9. Conclusion: The method is simple and highly reproducible

and can provide scientific basis for the quality control of Shenxuebao mixture.