Objective: To explore intervention effect of Shiquan Dabu Tang-medicated serum on proteins of Wnt/β-catenin signal transduction pathway in lung adenocarcinoma cisplatin-resistant cell lines of A549/DDP. Method: SD rats were randomly divided into 4 groups:high dose(65 g·kg-1

10 mL·kg-1) group

medium dose(32.5 g·kg-1

10 mL·kg-1) group

low dose(16.25 g·kg-1

10 mL·kg-1) group and blank control group(normal saline

10 mL·kg-1). A549/DDP cells were divided into blank serum group

high

medium and low dose groups of drug-medicated serum. MTT assay was used to determine 50% inhibition concentraction(IC50)values of cisplatin. Western blotting was adopted to detect the protein expression of glycogen synthase kinase 3β(GSK-3β)

p-GSK-3βser9

β-catenin and survivin. Result: With the increase in the concentration of medicated serum

IC50 values of cisplatin(DDP) in A549/DDP cells decreased

especially in mediummedium and high groups(P<0.01). Shiquan Dabu Tang-medicated serum had no effect on GSK-3β protein expression in A549/DDP cytoplasm

but can significantly down-regulate p-GSK-3βser9protein expression and total β-catenin and survivin protein expression in cytoplasm

particularly in medium and high groups(P<0.01). Conclusion: Shiquan Dabu Tang can lower the expression levels of cytoplasmic p-GSK-3βser9

total β-catenin and surviving proteins

so as to influence Wnt/β-catenin signal transduction pathway and sensitize cisplatin resistance.