Objective:To preliminarily evaluate the effect of 17 main components from Guizhi Fuling capsule on human leiomyoma cell proliferation and dysmenorrhea. Method:Human leiomyome cells were grown in 96-well plates and treated with 17 main components (25 mg·L-1) for 48 h. There were 6 complex wells for each group. Then Hoechst 33342/Propidium iodide(PI) double staining method was used to analyze the effect of the 17 main components on human leiomyome cell

and the anti-dysmenorrhea bioactivity of the 17 components was evaluated by the model Prostag landin F2α(PGF2α)-induced contraction of isolated mouse uterine

with 10 parallel tests for each group. Result:Compared with control group

quercetin

cinnamyl aldehyde

paeonol

pachymic acid

dehydrotumulosic acid

albiflorin and paeoniflorin inhibited human leiomyoma cell proliferation

with significant difference (P<0.05

P<0.01). Compared with control group

gallic acid

paeoniflorin

paeonol

laetrile

benzoylpaeoniflorin and 1

2

3

4

6-O-pentagalloylglucose significantly inhibited contraction of isolated mouse uterine (P<0.01). Conclusion:Quercetin

cinnamyl aldehyde

paeonol

pachymic acid

dehydrotumulosic acid

albiflorin and paeoniflorin in Guizhi Fuling capsule may be active ingredients for treating hysteromyoma

while gallic acid

paeoniflorin

paeonol

laetrile

benzoylpaeoniflorin and 1

2

3

4

6-O-pentagalloylglucose in Guizhi Fuling capsule may be active ingredients for treating primary dysmenorrhea.