绵马贯众不同萃取部位的抑菌活性

刘计权; 谢树莲; 李洋洋; 阴宇

doi:10.13422/j.cnki.syfjx.2016030137

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绵马贯众不同萃取部位的抑菌活性

药理 | 更新时间：2024-01-04

- 绵马贯众不同萃取部位的抑菌活性
- Antibacterial Activities of Different Extract Fractions from Dryopteridis Crassirhizomatis Rhizoma
- 中国实验方剂学杂志 2016年22卷第3期页码：137-142
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省自然科学基金项目(2011011036)
- DOI：10.13422/j.cnki.syfjx.2016030137
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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刘计权, 谢树莲, 李洋洋, 等. 绵马贯众不同萃取部位的抑菌活性[J]. 中国实验方剂学杂志, 2016,22(3):137-142.

LIU Ji-quan, XIE Shu-lian, LI Yang-yang, et al. Antibacterial Activities of Different Extract Fractions from Dryopteridis Crassirhizomatis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 137-142.
刘计权, 谢树莲, 李洋洋, 等. 绵马贯众不同萃取部位的抑菌活性[J]. 中国实验方剂学杂志, 2016,22(3):137-142. DOI： 10.13422/j.cnki.syfjx.2016030137.

LIU Ji-quan, XIE Shu-lian, LI Yang-yang, et al. Antibacterial Activities of Different Extract Fractions from Dryopteridis Crassirhizomatis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 137-142. DOI： 10.13422/j.cnki.syfjx.2016030137.

摘要

目的:研究绵马贯众不同萃取部位的抑菌活性。方法:以70%乙醇冷浸72 h制备绵马贯众粗提物

液-液分离法制备石油醚、三氯甲烷、乙酸乙酯、正丁醇和水5个部位萃取物

用K-B纸片扩散法对大肠埃希菌、金黄色葡萄球菌、枯草杆菌、酵母菌、黄曲霉和黑根霉6种供试菌株的抑菌活性进行研究。结果:绵马贯众5个萃取部位均表现出一定的抑菌效果

且随着用药量的增加其抑菌活性总体上呈现出增强的趋势

其中以三氯甲烷萃取部位和乙酸乙酯萃取部位的抑菌活性较大

水萃取部位的抑菌活性最小。结论:不同部位对供试菌株的抑菌活性不同。

Abstract

Objective: To study the antibacterial activities of different extract parts from Dryopteridis Crassirhizomatis Rhizoma. Method: The crude extracting of Dryopteridis Crassirhizomatis Rhizoma was extracted in 70% ethanol for 72 h

and the extracts of five fractions (petroleum

chloroform

EtoAc

n-BuoH and water) were prepared with liquid-liquid separation method. The antibacterial activities of different fractions on six strains (Escherichia coli

Staphylococcus aureus

Bacillus subtilis

Saccharomyces cerevisiae

Aspergillus flavus and Rhizopus nigricans) were studied by K-B paper dispersion. Result: All five parts had certain antibacterial effects on test strains

and the overall antibacterial activities were increase with the increase of the drug dose. The chloroform and EtoAc fractions had the stronger antibacterial activities

and the water fraction had the minimum antibacterial activities. Conclusion: Different fractions had different antibacterial activities on test strains.

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