Objective: To observe the therapeutic effect of Jishen capsule in models of ADR nephropathy rats by regulating cytokines

and explore the mechanism of action. Method: The models of ADR rats were established by twice intravenous injection of adriamycin and randomized into model group

positive ambrette capsule group (1.88 g·kg-1)

Jishen capsule high

medium and low dose groups (2.25

1.13

0.56 g·kg-1)

and a normal group. 10 rats was included in each group. Seven days later

drug administration was started. 24 hour urine protein quantity was examined in each group on day 7

14

and 28. Blood biochemical indicators of total protein (TP)

triglyceride (TG)

total cholesterol (TC)

albumin (Alb)

serum creatinine (SCr)

and urea nitrogen (BUN) were detected. Serum tumor necrosis factor-α (TNF-α)

interleukin-8 (IL-8)

nuclear factor-κB (NF-κB)

transforming growth factor-β1(TGF-β1) were measured by ELISA. Activity of superoxide dismutase (SOD)

malonaldehyde (MDA) were detected in the serum and renal pathological changes were observed by HE staining. Result: Compared with the normal group

24 h proteinuria

TG

TC

SCr

BUN contents in serum and levels of TNF-α

IL-8

NF-κB

TGF-β1

MDA were significantly increased

while TP

Alb contents and serum SOD activity were significantly reduced in model group (P<0.01). Jishen capsule high-dose group could significantly reduce 24 h proteinuria of the ADR nephropathy rats

decrease the contents of TG

TC

SCr

BUN (P<0.05

P<0.01) and significantly increase the level of TP

Alb in serum (P<0.05

P<0.01)

significantly reduce the cytokines level of TNF-α

IL-8

NF-κB

TGF-β1 in serum (P<0.05

P<0.01)

increase the activity of serum SOD and decrease serum MDA level (P<0.05)

relieve the pathological changes of renal tissues and improve renal function. Conclusion: Jishen capsules can treat the rats with ADR nephropathy by regulating cytokines levels of TNF-α

IL-8

NF-κB

and TGF-β1 in serum

with protective effects on renal tissues.