Objective: To preliminarily interpret the compatibility of ginseng and flower of Pueraria lobata in chemical and pharmacodynamical levels

thus provide theoretical basis for its clinical application. Method: Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry(RRLC-Q-TOF-MS) has been applied to identify and analyze the chemical components of the flower of Pueraria lobata and Ginseng Radix et Rhizoma before and after compatibility. 0.1% formic acid water (A)-acetonitrile (B) was used as the mobile phase for gradient elute (0-12 min

15%B;12-20 min

15%-50%B). The flow rate was set to 0.4 mL · min-1 and the sample injection volume was 5 μL. Principal component analysis (PCA) was used to find the differences in chemical components before and after compatibility

and pharmacodynamical experiment was conducted for the compatibility of these two herbs. Result: The chemical components of flower of Pueraria lobata and Ginseng Radix et Rhizoma were identified before and after the compatibility;in negative ion mode of mass spectrometry

irisolidon

6"-O-xylosyl-glycitin

ginsenoside Rg1

and ginsenoside Rf contents in the compatibility mixture were significantly increased. By PCA analysis

the components before and after compatibility can be clearly distinguished. In the enzymatic activity assay

flower of Pueraria lobata had the acceleration effect for enzyme reaction

and three indicators had significant changes during the in vivo experiments. Conclusion: Compatibility of Ginseng Radix et Rhizoma and flower of Pueraria lobata has significant effect on alleviating hangover and protecting liver in vitro and in vivo

and the content and changes of chemical components have been detected

providing theoretical basis for its clinical application and development.