Objective: To optimize the screening

sterilization and culture medium for explants of Trillium tschonoskii

reduce the pollution rate and browning rate

and establish an aseptic tissue culture system of T. tschonoskii. Method: Most suitable explants were selected from ovary

leaves

stems

underground rhizomes and root tips of T. tschonoskii

and the effect of a variety of drug liquid alternating sterilization and addition of antibiotics in medium were studied on the contamination rate. VitC(VC)

polyvinyl pyrrolidone(PVP) and activated carbon were added to the medium to study their effect on reducing the browning rate. Result: The underground rhizome of T. tschonoskii was the most optimum explants. It could be alternately sterilized with ultraviolet radiation for 30 min

800 times of carbendazim soaking for 30 min

and processed with 0.2%HgCl2 for 20 min. The medium supplemented with 100 mg · L-1 ampicillin and 1.0 g · L-1activated carbon could reduce the contamination rate and browning rate of the rhizome tissue culture. Conclusion: The rhizome sterile culture system of T. tschonoskii has been established with low contamination rate and browning rate. It can provide reference for tissue culture of other rhizome-plants.