大黄硝石滴丸HPLC多波长指纹图谱及多成分含量测定

药雅俊; 孔祥鹏; 裴妙荣

doi:10.13422/j.cnki.syfjx.2016060030

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大黄硝石滴丸HPLC多波长指纹图谱及多成分含量测定

化学与分析 | 更新时间：2024-01-04

- 大黄硝石滴丸HPLC多波长指纹图谱及多成分含量测定
- HPLC Multi-wavelength Chromatographic Fingerprints and Multi-component Content Determination of Dahuang Xiaoshi Dropping Pill
- 中国实验方剂学杂志 2016年22卷第6期页码：30-33
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省国际科技合作项目(2013081064-4);2014年山西省技术创新项目;山西中医学院博士基金项目
- DOI：10.13422/j.cnki.syfjx.2016060030
  中图分类号： O657.72;R286.0
- 纸质出版日期：2016
- 稿件说明：
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药雅俊, 孔祥鹏, 裴妙荣. 大黄硝石滴丸HPLC多波长指纹图谱及多成分含量测定[J]. 中国实验方剂学杂志, 2016,22(6):30-33.

YAO Ya-jun, KONG Xiang-peng, PEI Miao-rong. HPLC Multi-wavelength Chromatographic Fingerprints and Multi-component Content Determination of Dahuang Xiaoshi Dropping Pill[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 30-33.
药雅俊, 孔祥鹏, 裴妙荣. 大黄硝石滴丸HPLC多波长指纹图谱及多成分含量测定[J]. 中国实验方剂学杂志, 2016,22(6):30-33. DOI： 10.13422/j.cnki.syfjx.2016060030.

YAO Ya-jun, KONG Xiang-peng, PEI Miao-rong. HPLC Multi-wavelength Chromatographic Fingerprints and Multi-component Content Determination of Dahuang Xiaoshi Dropping Pill[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 30-33. DOI： 10.13422/j.cnki.syfjx.2016060030.

摘要

目的: 建立大黄硝石滴丸HPLC多波长指纹图谱

并对栀子苷、盐酸小檗碱、西红花苷同时进行含量测定

以期对其质量进行控制。方法: 采用国家药典委员会颁布的"中药色谱指纹图谱相似度评价系统"2004A对10批大黄硝石滴丸样品进行评价

建立对照指纹图谱

并对其主要活性成分进行含量测定。结果: 分别在238

345

440 nm下标定了6

8个共有峰

指纹图谱相似度均>0.9。结论: 大黄硝石滴丸的多波长指纹图谱特征性和专属性强

通过栀子苷、盐酸小檗碱、西红花苷的测定

可以有效地对大黄硝石滴丸的质量进行控制。

Abstract

Objective: To establish the multi-wavelength chromatographic fingerprints of Dahuang Xiaoshi dropping pill based on chromatographs of HPLC-PDA

and determine the contents of geniposide

berberine hydrochloride and crocin. Method: The multi-wavelength chromatographic fingerprints and determination 10 batches of homemade pills were performed

with geniposide

berberine hydrochloride and crocin as reference peak. The Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (2004A) was used for data analysis. Result: As a result

six common peaks were pointed out at 238 nm

three common peaks were pointed out at 345 nm

and eight common peaks were pointed out at 440 nm. Dahuang Xiaoshi dropping pill from different batches showed a similarity of above 0.9 in HPLC fingerprint chromatogram. Conclusion: This method is highly characteristic and specific. The multi-wavelength HPLC fingerprint and the determination of geniposide

berberine hydrochloride and crocin can be used for the quality control.

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