Objective: To establish the HPLC fingerprints of flavonoids in Ophiopogonis Radix of Zhejiang and simultaneously determine the contents of methylophiopogonanone A and methylophiopogonanone B. Method: The chromatographic method was carried out on Kromasil 100-5 C18 column (4.6 mm×250 mm

5 μm) using acetonitrile-0.1%phosphoric acid (58 : 42) as the mobile phase. The flow rate was 1.0 mL · min-1;and the column temperature was 30 ℃. Result: The HPLC fingerprints of Ophiopogonis Radix of Zhejiang were established and 7 common peaks were defined in the HPLC fingerprints. The similarity among different batches was above 0.9.By HPLC-MS analysis and comparison with standard reference substance

No. 1 peak was determined as methylophiopogonanone A and No. 2 peak was determined as methylophiopogonanone B. In the quantitative analysis of these two compounds

we found that the linear range was 31.47-786.8 ng for methylophiopogonanone A (R2=1.000 0) and 53.05-1 326.25 ng for methylophiopogonanone B (R2=1.000 0)

and the average recovery rate (n=6) was 101.5% for methylophiopogonanone A and 103.0% for methylophiopogonanone B. Conclusion: The HPLC fingerprint method and content determination method are simple

accurate with good reproducibility

and can be applied in the quality control of Ophiopogonis Radix of Zhejiang.