Objective: To establish the HPLC fingerprint chromatogram of Crassocephalum crepidioides coupled with chemometrics means for the quality control of multi-batches of medicinal material. Method: The separation was developed on C18 column (Phenomenex

4.6 mm×250 mm

5 μm) by gradient elution with acetonitrile-water (both containing 0.1% phosphoric acid) as mobile phase at a flow rate of 1.0 mL·min-1

the detection wavelength at 328 nm and column temperature at 30℃. The HPLC fingerprint chromatogram of C. crepidioides was set up. The quality of 13 batches of medicinal material was evaluated by similarity assay as well as cluster analysis. Result: The establish HPLC fingerprint chromatogram of C. crepidioides was specfic

precise

reproduccible and stable. The similarities of 13 batches of C. crepidioides were more than 0.97. Further cluster analysis was carried out for the quality consistency of 13 batches of C. crepidioides and accordingly they were devided into 4 clusters. Conclusion: With the cluster analysis

the HPLC fingerprint chromatogram C. crepidioides provides a method for qulity control of C. crepidioides.