高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分

梁咏倩; 余积聪; 宋粉云

doi:10.13422/j.cnki.syfjx.2016100080

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高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分

化学与分析 | 更新时间：2024-01-04

- 高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分
- Simultaneous Determination of Three Kinds of Glycosides Components in Juhong Huatan Wan by HPLC with Gradient Elution and Three Wavelength Detection
- 中国实验方剂学杂志 2016年22卷第10期页码：80-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省自然科学基金项目(5002841)
- DOI：10.13422/j.cnki.syfjx.2016100080
  中图分类号： O657.72;R286.0
- 纸质出版日期：2016
- 稿件说明：
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梁咏倩, 余积聪, 宋粉云. 高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分[J]. 中国实验方剂学杂志, 2016,22(10):80-83.

LIANG Yong-qian, YU Ji-cong, SONG Fen-yun. Simultaneous Determination of Three Kinds of Glycosides Components in Juhong Huatan Wan by HPLC with Gradient Elution and Three Wavelength Detection[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(10): 80-83.
梁咏倩, 余积聪, 宋粉云. 高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分[J]. 中国实验方剂学杂志, 2016,22(10):80-83. DOI： 10.13422/j.cnki.syfjx.2016100080.

LIANG Yong-qian, YU Ji-cong, SONG Fen-yun. Simultaneous Determination of Three Kinds of Glycosides Components in Juhong Huatan Wan by HPLC with Gradient Elution and Three Wavelength Detection[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(10): 80-83. DOI： 10.13422/j.cnki.syfjx.2016100080.

摘要

目的: 建立高效液相色谱-三波长检测-梯度洗脱法同时测定橘红化痰丸中3种苷类成分含量的方法。方法: 采用Diamonsil-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%磷酸水溶液进行梯度洗脱

流速1.0 mL·min-1

柱温30℃

进样量10 μL

检测波长分别210 nm(苦杏仁苷)

276 nm(甘草苷)和283 nm(柚皮苷)。结果: 苦杏仁苷在25.0~175.0 mg·L-1具有良好的线性关系(r=0.999 1)

苦杏仁苷平均回收率为101.5%

RSD 1.9%;甘草苷在2.0~50.0 mg·L-1具有良好的线性关系(r=0.999 5)

甘草苷平均回收率为100.8%

RSD 1.5%;柚皮苷在6.3~126.0 mg·L-1具有良好的线性关系(r=0.999 2)

柚皮苷平均回收率为101.7%

RSD 1.6%。结论: 该法操作简单

重复性好

测定结果准确

可用于更好地控制该制剂的质量。

Abstract

Objective: A high performance liquid chromatographic method with gradient elution and three wavelength detection was developed for the simultaneous determination of amygdalin

liquiritin and naringin in Juhong Huatan Wan. Method: The separation was performed on a Diamonsil-C18 column with methanol and 0.1% phosphoric acid as the mobile phase of gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelengths were set at 210

276

283 nm. Result: The linear range of amygdalin was 25.0-175.0 mg·L-1(r=0.999 1). The average recovery for amygdalin was 101.5%

precision of the method was 1.9%(RSD). The linear range of liquiritin was 2.0-50.0 mg·L-1(r=0.999 5). The average recovery for liquiritin was 100.8%

precision of the method was 1.5%(RSD). The linear range of naringin was 6.3-126.0 mg·L-1(r=0.999 2). The average recovery for naringin was 101.7%

precision of the method was 1.6%(RSD). Conclusion: The method is simple

accurate and reproducible. It is a good method for the quality control of the preparation.

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