Objective: To investigate the chemical components from leaves of Gardenia jasminoides

in order to provide a scientific basis for its development and utilization. Method: The compounds were isolated and purified from 95%ethyl acetate fraction and water extract of leaves of Gardeniae Fructus that were collected from Lishui Country

Nanjing. Separation techniques

such as column chromatography over Sephadex LH-20

macroporous resin and C18

were adopted. Structures were identified through spectroscopic techniques(1H-NMR

13 C-NMR) and physicochemical properties. Result: Eight compounds were isolated and identified as β-gardiol (1)

α-gardiol (2)

β-sitosterol (3)

kaempferol (4)

quercetin (5)

geniposide (6)

gardenoside (7) and β-daucosterol(8). Conclusion: Compounds 1

2

4-7 are isolated from leaves of G. jasminoidesfor the first time.