Objective: To study the effect of Guizhou Zedoary Turmeric Oil on cell proliferation of human rectal cancer cells SW1463

in order to detect the effect of expressions of immune factor interleukin(IL)-2 and transforming growth factor-β1 (TGF-β1)

and the effect of FasL

Capase-3 and matrix metalloproteinase-9 (MMP-9) protein expressions. Method: The steam distillation method was used to extract volatile oil from Zedoary Turmeric Oil with concentrations of 40

80

120

160

200

240

280 mg·L-1 to intervene SW1463 cells for 24

48

72 h. Thiazolyl blue tetrazolium bromide (MTT) was used to detect the effect on cells proliferation inhibition rate. Giemsal staining was used to observe the effect of different concentrations of Zedoary Turmeric Oil on the morphology of SW1463 cells apoptosis. Enzyme linked immunosorbent assay (ELISA) method was adopted to detect IL-2 and TGF-β1 expression. Western blot assay was used to detect the expressions of Capase-3

MMP-9 and FasL. Result: The MTT assay showed that the oil had a certain inhibitory effect on cell SW1463 proliferation in a time and dose-dependent manner. The half maximal inhibitory concentration (IC50) was 128.49 mg·L-1. With the increase of drug concentrations

microscope observation the cell became smaller and round; Giemsal staining showed typical apoptotic morphological features. The expression of IL-2 and TGF-β1 contents decreased with increase of oil concentrations

single factor analysis showed significant differences in IL-2 expression (P < 0.05) and TGF-β1 expression (P < 0.01). Western blot showed up-regulations in FasL and Capase-3 proteins

but down-regulation in MMP-9 protein

after Zedoary Turmeric Oil processed SW1463 cells for 24 h. Conclusion: Zedoary Turmeric Oil can significantly inhibit SW1463 cells. Therefore the expression of Caspase-3 was negatively correlated with MMP-9.The mechanism may be related to the up-regulation of FasL-induced cellular immune factors.