HE Xiao-ying, HUANG Yan-ping, TANG Li-li, et al. Quality Spectificationg of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 73-77.
HE Xiao-ying, HUANG Yan-ping, TANG Li-li, et al. Quality Spectificationg of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 73-77. DOI: 10.13422/j.cnki.syfjx.2016150073.
Objective: To establish the quality standard of Aeschynanthus bracteatus
and provide scrientific basis for its quality control. Method: Empirical and microscopic identification methods were adopted to observe morphological characteristics
microscopic characteristics
miosture content
ash content and ethanol-soluble extracts were determined by empirical method and referring to the method in Chinese Pharmacopoeia (2015). TLC and HPLC were used for qualitative identification and quantitative analysis of 5
7-dihydroxy-6
4'-methoxyfldvone in A. bracteatus. Result: The macroseopical identification
microscopic identification and TLC method had the specificity. The sample size of 5
7-dihydroxy-6
4'-methoxyfldvone was between 0.108 2 μg to 1.082 μg (r=0.999 9) with an average recovery rate of 99.7% (RSD 1.1%). The content of 5
7-dihydroxy-6
4'-methoxyfldvone in 6 batches of samples was between 0.62% to 0.88%. Moisture content in A. bracteatus was between 8.9% to 11.0%; total ash was between 3.9% to 7.4%; ethanol-soluble extracts content was between 11.0% to 15.9%. Conclusion: The established method can be used for quality control of Aeschynanthus bracteatus.