Objective: To investigate the inhibitive effect of Gehua Jiecheng decoction on tumor growth of transplanted H22 in BALB/c mice and expressions of adenomatous polyposis coli (APC) protein in tumor tissues. Method: Thirty-four male BALB/c mice were randomly divided into four groups

namely blank group

model group and Gehua Jiecheng decoction of high and low dose(40

20 g·kg-1) groups. Except for the blank control group

the other groups were included in the preparation of the transplanted liver cancer mouse model. At the 2nd day after the modeling

the mice were given drugs

the blank control group and the model group were given NS

ig

and Gehua Jiecheng decoction high and low dose groups were given the same volume of decoction

ig

with the dosage 0.2 mL for each. After 12 days

all mice were sacrificed. Their tumor lumps were collect to weigh and calculate the inhibition rate and make pathological sections. Spleen and thymus were collected to calculate the spleen index and thymus index

enzyme-linked immunosorbent assay (ELISA) was used to examine interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) content in peripheral blood after blood was collected and centrifuged. Immunohistochemistry was used to examine the expression of APC

Western blot method was adopted to detect the expression of APC protein expression. Result: Compared with the model control group

the inhibition rate of Gehua Jiecheng decoction high and low dose groups were 37.05%and 16.51%(P<0.05

P<0.01)

the model group showed high expression of APC protein

Gehua Jiecheng decoction high and low dose groups showed significantly lower APC expressions than model group (P<0.05

P<0.01). Conclusion: Under a certain dose range

Gehua Jiecheng decoction can inhibit the growth of liver cancer in mice tumor blocks in a dose-effect manner

and regulate and control cancer suppressor gene APC

which may one of Gehua Jiecheng decoction's mechanisms for inhibiting liver cancer.