苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响

付伟; 孙雄杰; 李水清; 刘艳菊; 陈祥胜; 杨建锟

doi:10.13422/j.cnki.syfjx.2016190019

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苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响

饮片工艺与质量研究专题 | 更新时间：2024-01-04

- 苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响
- Effects of Atractylodis Rhizoma Before and After Being Deep-fried on Contents of Aquaporin 2 and Aquaporin 3 in Model Rats with Syndrome of Damp Retention in Middle-jiao
- 中国实验方剂学杂志 2016年22卷第19期页码：19-22
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81541080）;国家科技部科技基础性专项（2014FY111100-2）
- DOI：10.13422/j.cnki.syfjx.2016190019
  中图分类号： R-332;R285.5
- 纸质出版日期：2016
- 稿件说明：
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付伟, 孙雄杰, 李水清, 等. 苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响[J]. 中国实验方剂学杂志, 2016,22(19):19-22.

FU Wei, SUN Xiong-jie, LI Shui-qing, et al. Effects of Atractylodis Rhizoma Before and After Being Deep-fried on Contents of Aquaporin 2 and Aquaporin 3 in Model Rats with Syndrome of Damp Retention in Middle-jiao[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 19-22.
付伟, 孙雄杰, 李水清, 等. 苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响[J]. 中国实验方剂学杂志, 2016,22(19):19-22. DOI： 10.13422/j.cnki.syfjx.2016190019.

FU Wei, SUN Xiong-jie, LI Shui-qing, et al. Effects of Atractylodis Rhizoma Before and After Being Deep-fried on Contents of Aquaporin 2 and Aquaporin 3 in Model Rats with Syndrome of Damp Retention in Middle-jiao[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 19-22. DOI： 10.13422/j.cnki.syfjx.2016190019.

摘要

目的：探讨苍术炒焦前后对湿阻中焦模型大鼠水通道蛋白2（AQP2）和AQP3含量的影响，为苍术炒焦机制研究提供参考。方法：将动物分成空白组，湿阻中焦模型组，生苍术高、中、低剂量组（3 459.2，1 729.6，864.8 mg·kg-1）及焦苍术高、中、低剂量组（剂量同生品）和香砂胃苓丸阳性组（1 729.6 mg·kg-1），连续灌胃给药7 d。利用紫外分光光度法和酶联免疫法分别检测给药前后大鼠尿液中AQP2含量和结肠黏膜中AQP3含量。结果：与空白组比较，模型组尿液中AQP2含量增加，结肠黏膜中AQP3含量降低，两者间的差异均具有极显著性意义。与模型组比较，各给药组大鼠尿液中AQP2含量均降低，且生品组AQP2含量较焦品组低；结肠黏膜中AQP3含量均增加，且焦品组含量高于生品组。结论：苍术的生品与焦品均有祛湿止泻作用，生苍术祛湿作用强于焦品，焦苍术止泻作用强于生品。

Abstract

Objective: To explore effects of Atractylodis Rhizoma before and after being deep-fried on contents of aquaporin 2 (AQP2) and AQP3 in model rats with syndrome of damp retention in middle-jiao and provide an reference for mechanism research of Atractylodis Rhizoma before and after being deep-fried. Method: Animals were divided into the blank group

the damp retention in middle-jiao model group

the high

middle and low dose (3 459.2

1 729.6

864.8 mg·kg-1) group of raw products of Atractylodis Rhizoma

the high

middle and low dose (3 459.2

1 729.6

864.8 mg·kg-1) group of deep-fried products of Atractylodis Rhizoma

Xiangsha Weiling pills positive group (1 729.6 mg·kg-1)

and rats were intragastric administration for seven days.Contents of AQP2 in urine and AQP3 in colonic mucosa were detected by UV and euzymelinked immunosorbent assay respectively before and after administration. Result: Compared with the blank group

AQP2 content increased and AQP3 content lowered in the model group; difference between these two groups were statistically significant.Compared with the model group

AQP2 content in administration group were declined and this index in raw products was lower than those of deep-fried group;the content of AQP3 in colonic mucosa rose dramatically and the deep-fried group was higher than that of raw product group. Conclusion: Both of raw and deep-fried products have effects of clearing damp and antidiarrheal.Function of raw products is stronger than deep-fried products in terms of clearing damp effect.However

in the aspect of antidiarrhoea

the role of deep-fried products is stronger than raw products.

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