当归补血汤对腹膜透析相关性大鼠腹膜功能衰竭的影响

杜丽东; 吴国泰; 王霞; 张秀秀; 邵晶; 王瑞琼; 任远

doi:10.13422/j.cnki.syfjx.2016200112

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当归补血汤对腹膜透析相关性大鼠腹膜功能衰竭的影响

药理 | 更新时间：2024-01-04

- 当归补血汤对腹膜透析相关性大鼠腹膜功能衰竭的影响
- Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats
- 中国实验方剂学杂志 2016年22卷第20期页码：112-116
- 作者机构：
- 作者简介：
- 基金信息：
  
  甘肃省自然科学基金项目（1310RJZA090）;甘肃中医学院中青年基金项目（BH2012-023）
- DOI：10.13422/j.cnki.syfjx.2016200112
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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杜丽东, 吴国泰, 王霞, 等. 当归补血汤对腹膜透析相关性大鼠腹膜功能衰竭的影响[J]. 中国实验方剂学杂志, 2016,22(20):112-116.

DU Li-dong, WU Guo-tai, WANG Xia, et al. Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 112-116.
杜丽东, 吴国泰, 王霞, 等. 当归补血汤对腹膜透析相关性大鼠腹膜功能衰竭的影响[J]. 中国实验方剂学杂志, 2016,22(20):112-116. DOI： 10.13422/j.cnki.syfjx.2016200112.

DU Li-dong, WU Guo-tai, WANG Xia, et al. Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 112-116. DOI： 10.13422/j.cnki.syfjx.2016200112.

摘要

目的：研究当归补血汤（Danggui Buxue Tang，DBT）对腹膜透析相关性大鼠腹膜功能衰竭的影响，并探讨相关的作用机制。方法：SD大鼠ip 4.25%高糖透析液100 mL·kg-1，连续40 d，在第8，10，12天ip大肠埃希菌脂多糖5 mg·kg-1，建立腹膜功能衰竭大鼠模型；造模大鼠随机分为4组，每组10只，分别为模型组，贝那普利组（2.1 mg·kg-1），DBD高、低剂量组（12，6 g·kg-1），另设正常组，各组大鼠造模的同时ig给药；实验期间观察各大鼠活动状态，第40天末次ip透析液2 h后进行腹膜平衡实验，计算超滤量（UF），检测血清肌酐（PCr）和尿素氮（PUN），腹透液中肌酐（DCr），尿素氮（DUN），葡萄糖（DGlu）及4.25%透析液中的葡萄糖（D0Glu），计算腹膜转运功能参数（DCr/PCr，DUN/PUN，DGlu/D0Glu），取大鼠壁层腹膜采用苏木素-伊红（HE）观察组织形态变化，并用免疫组化法检测转化生长因子-β1（TGF-β1）蛋白的表达。结果：与正常组比较，模型组大鼠PCr和PUN均显著升高（P<0.01），DCr和DGlu显著降低，DUN显著升高（P<0.01），UF，DCr/PCr，DUN/PUN及DGlu/D0Glu均显著降低（P<0.01），腹膜间皮细胞受损明显，腹膜TGF-β1表达增高（P<0.01）；与模型组比较，DBT高、低剂量组PCr和PUN均显著降低（P<0.05），DGlu显著升高（P<0.01），DBT低剂量组DUN显著升高（P<0.05），DBT高剂量组大鼠UF显著升高（P<0.05），DBT高、低剂量组DCr/PCr，DUN/PUN及DGlu/D0Glu均显著升高（P<0.01）；DBT高、低剂量组大鼠腹膜组织形态明显改善，且TGF-β1表达显著降低（P<0.05，P<0.01）。结论：DBT能抑制腹膜透析相关性大鼠腹膜功能衰竭，保护腹膜结构。

Abstract

Objective: To observe the protective effects of Danggui Buxue Tang (DBT) on peritoneal function failure induced by peritoneal dialysis in rats. Method: Peritoneal function failure model in rats were established by intraperitoneally injecting 4.25%of high-glucose peritoneal dialysate (100 mL·kg-1) for 40 d and administering lipopolysaccharide (LPS

5 mg·kg-1) at 8

12 d. The rats were randomly divided into four groups

with 10 rats in each group. They were respectively model group

Benazepril Hydrochloride group (BH 2.1 mg·kg-1)

DBT high-dose and low-dose groups (12

6 g·kg-1). The other 10 rats were randomly chosen as normal control group. During modeling

rats in each group were given drugs

ig. During the experiment

body weights and general states of all rats were detected. At 40 d

the two hour later after intraperitoneal administration with dialysate

the peritoneal equilibration test was performed to detect the ultrafiltration volume (UF

PCr

PUN

DCr

DUN and DGlu)

and calculate peritoneal transport function parameters (DCr/PCr

DUN/PUN

DGlu/D0Glu). Parietal peritoneum tissues of rats were stained with hematoxylin-eosin (HE) to observe the changes of peritoneal morphology

the transforming growth factor-β1(TGF-β1) expression in parietal peritoneum of rats WAS observed by Immunohistochemical method. Result: Compared with normal group

in the model group

PCr

PUN

DUN were increased (P<0.01)

the levels of DCr

DGlu

DCr/PCr

DUN/PUN and DGlu/D0Glu were decreased (P<0.01)

peritoneal mesothelioma cells were significantly impaired

and the expression of TGF-β1 was also increased (P<0.01). Compared with model group

in DBT high-dose and low-dose groups

PCr

PUN were decreased (P<0.05)

the levels of DGlu were significantly increased (P<0.01)

DCr/PCr

DUN/PUN and DGlu/D0Glu were significantly increased (P<0.05

P<0.01)

with obvious improvement in peritoneal histomorphology and decrease in TGF-β1 expression (P<0.05

P<0.01). Conclusion: DBT shows the protective effects on peritoneal function failure induced by peritoneal dialysis in rats.

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