Objective: To provide the scientific basis for the utilization and development of Lindernia ruellioides by formulating the quality control standards for it. Method: The characteristics and microscopic identification of L.ruellioides were studied. With verbascoside as the reference substance

TLC was applied in the qualitative identification of it. Moisture

total ash

acid insoluble ash and extractives in L.ruellioides was determined based on the approaches in the appendix of Chinese Pharmacopoeia published in 2015

and the content of verbascoside was analyzed by HPLC. Result: The characteristics and microscopic identification of L.ruellioides were described

with stable and reliable identification characteristics. The spots in TLC were clear

with a good separation. The limits for moisture

total ash and acid insoluble ash in L.ruellioides shall be less than 13.00%

21.00%and 10.00%

respectively

and the limits for the extractives and the content of verbascoside shall be more than 15.00%and 0.20%. Conclusion: The method established is simple

accurate and reliable

and the standard limit is reasonable

whic can be used to control the quality of L.ruellioides.