Objective: To study the chemical constituents in Fritillaria Ussuriensis Bulbus water solution

extract Fritillaria Ussuriensis Bulbus by the method of water solution

and establish LC-ESI-TRAP-MS (LC-MSn) method to analyze the chemical components in Fritillaria Ussuriensis Bulbus water solution. Method: LC-MSn method was used to determine the chemical components in Fritillaria Ussuriensis Bulbus on Agilent ZORBAX Extend-C18 column (2.1 mm×50 mm 1.8 μm)

with acetonitrile-0.1%ammonia as the mobile phase for gradient elution. Data were collected with posi tive ESI source. Structures of the components were elucidated by comparing the total ion chromatogram

multi-stage mass spectrogram

and mass spectrometry fragment information between Fritillaria Ussuriensis Bulbus water solution and control products and by referring to the compound standard mass spectrometry fragmentation rules. Result: The 22 peaks were found in this study; 19 alkaloid components were identified; and 3 unknown alkaloid components were found from Fritillaria Ussuriensis Bulbus water solution. Conclusion: LC-MSn method is suitable for the routine analysis of alkaloid components in Fritillaria Ussuriensis Bulbus and also can help to improve the understanding of its pharmacodynamic action

providing experimental and theoretical basis for the study of chemical components and quality control of Fritillaria Ussuriensis Bulbus.