广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响

刘瑶; 焦豪妍; 刘伟

doi:10.13422/j.cnki.syfjx.2016210142

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广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响

药理 | 更新时间：2024-01-04

- 广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响
- Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome
- 中国实验方剂学杂志 2016年22卷第21期页码：142-146
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省中医药局科研项目（20131279）
- DOI：10.13422/j.cnki.syfjx.2016210142
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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刘瑶, 焦豪妍, 刘伟. 广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响[J]. 中国实验方剂学杂志, 2016,22(21):142-146.

LIU Yao, JIAO Hao-yan, LIU Wei. Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 142-146.
刘瑶, 焦豪妍, 刘伟. 广藿香油对感染后肠易激综合征大鼠肠黏膜屏障的影响[J]. 中国实验方剂学杂志, 2016,22(21):142-146. DOI： 10.13422/j.cnki.syfjx.2016210142.

LIU Yao, JIAO Hao-yan, LIU Wei. Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 142-146. DOI： 10.13422/j.cnki.syfjx.2016210142.

摘要

目的：研究广藿香油对感染后肠易激综合征（PI-IBS）大鼠肠黏膜机械屏障和免疫屏障的保护作用。方法：将SD大鼠随机分为正常组，PI-IBS模型组、藿香正气液组、广藿香油低、中、高剂量组，每组8只。采用结肠灌注乙酸的方法建立PI-IBS大鼠模型。正常组和模型组ig生理盐水，藿香正气液组ig藿香正气液3.3 mL·kg-1，广藿香油低、中、高剂量组分别ig广藿香油2，3，4 g·kg-1，每日1次，共5 d。采用透射电镜观察结肠黏膜上皮细胞超微结构；采用酶联免疫吸附测定（ELISA）的方法检测各组大鼠血清二胺氧化酶（DAO）和血清免疫球蛋白A（SIgA）含量；采用免疫组化检测结肠肠黏膜细胞间黏附分子-1（ICAM-1）的表达。结果：PI-IBS大鼠结肠上皮细胞微绒毛稀疏，长短不一，有微绒毛断裂现象。广藿香油高剂量组对受损的上皮细胞有改善作用。PI-IBS组血清DAO含量高于正常组（P<0.05）。广藿香油高剂量组血清DAO含量低于PI-IBS组（P<0.05），与正常组比较，无显著性差异。PI-IBS组血清SIgA含量低于正常组（P<0.01）。藿香正气液组、广藿香油高剂量组血清SIgA含量与正常组之间无显著性差异。PI-IBS组ICAM-1蛋白表达IA值明显高于正常组（P<0.01）。广藿香油高剂量组ICAM-1蛋白表达IA值均明显低于模型组（P<0.05），与正常组比较，无显著性差异。结论：广藿香油通过修复受损的肠上皮细胞的超微结构，降低肠道通透性，保护肠黏膜机械屏障。通过促进SIgA分泌，抑制ICAM-1的表达，发挥对PI-IBS免疫屏障的调节作用。

Abstract

Objective: To protective and regulative effect Patchouli oil on intestinal mucosal mechanical barrier and immune barrier of rats with post-infectious irritable bowel syndrome(PI-IBS). Method: Sprague-Dawley rats were randomly divided into normal control group

PI-IBS model group

Huoxiang Zhengqi liquid group (HXZQ group)

low-dose of Patchouli oil

middle-dose of Patchouli oil and high-dose of Patchouli oil

with 8 rats in each group. The rat model of intracolonic instillation of acetic acid (4%)-induced colitis is set up. Control group and PI-IBS model group were treated with water

HXZQ group was treated with HXZQ(3.3 mL·kg-1). Low-dose of Patchouli oil

middle-dose of Patchouli oil and high-dose of Patchouli oil were treated with Patchouli oil (2

4 g·kg-1). After different treatment for 5 days

intestinal epithelial ultrastructure was analyzed by transmission electron microscopy (TEM)

the diamine oxidase (DAO) activity and SIgA content in serum was measured by ELISA assay. ICAM-1 were detected by immunohistochemistry. Result: Microvilli on the surface of the epithelial cells was sparse

uneven distribution and different length in PI-IBS model group. Recovery effect is obvious in high-dose group of Patchouli oil. Serum DAO content in model group was higher than it of the normal group (P<0.05). Compared with PI-IBS model group

serum DAO content in high-dose groups were significantly decreased (P<0.05). The difference between the normal group and high-dose groups was not statistically significant. Serum SIgA content in model group was lower than it of the normal group (P<0.01). The difference between the normal group and high-dose groups or HXZQ group was not statistically significant. ICAM-1 protein expression IA values in model group was higher than it of the normal group (P<0.01). Compared with PI-IBS model group

ICAM-1 protein expression IA values in high-dose groups were significantly decreased (P<0.05). The difference between the normal group and high-dose groups was not statistically significant. Conclusion: Patchouli oil can repair intestinal epithelial ultrastructure in rats with PI-IBS

reduce intestinal permeability

and then protect Intestinal mucosal mechanical barrier. Patchouli oil could promote SIgA

inhibit the expression of ICAM-1

thereby regulate immune barrier of rats with PI-IBS.

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