Objective: To establish HPLC method for the determination of kaempferol in Kaempferiae Rhizoma

analyze the contents of kaempferol in Kaempferiae Rhizoma from different origins

and provide reference for the evaluation of the medicinal quality. Method: ODS2 C18 (4.6 mm×150 mm

5 μm) was ised as chromatographic column

with methanol-0.4% phosphate (50:50) as the mobile phase; the flow rate was 1 mL·min-1

column temperature was room temperature; the detection wavelength was set at 367 nm; and the injection volume was 10 μL. Result: Kaempferol showed good linear relationship in the concentration range of 0.015 8-1.58 μg; the recovery rate was 97.8%

RSD of 1.6%. Conclusion: There was great difference in content of kaempferol in Kaempferiae Rhizoma from different origins. The established method is simple

accurate

repeatable

and can be used for quality control of Kaempferiae Rhizoma.