Objective: To discuss the change law of chemical constituents in Phellodendri Amurensis Cortex before and after being processed

and to provide the basis for the quality standard of its decoction pieces.Method: HPLC was performed by taking acetonitrile-0.4 mol·L-1 ammonium chloride solution as mobile phase

column temperture at 35℃

the flow rate of 1.0 mL·min-1 and the detection wavelength of 215 nm.Chemical constituents in Phellodendri Amurensis Cortex before and after being processed was analyzed and 7 main constituents were measured.Result: Among 10 batches of Phellodendri Amurensis Cortex decoction pieces

the contents of magnoflorine

phellodendrine

jatrorrhizine

palmatine

berberine

obaculactone and obakunone were 2.468-6.783

1.947-4.197

0.209-0.506

3.049-4.557

5.869-13.168

5.141-7.337

1.798-4.975 mg·g-1.After being processed

in addition to a sample

all the constituents reduced

among which alkaloids like berberine and palmatine

almost disappeard

however

obakunone and obaculactone existed.Among 9 batches of Phellodendri Amurensis Cortex decoction pieces

the contents of obaculactone and obakunone were 3.164-6.483

0.281-1.614 mg·g-1.Conclusion: The appearance and internal components of Phellodendri Amurensis Cortex after being processed obviously change.The quality standard

which applies to Phellodendri Amurensis Cortex

can't apply to charred Phellodendri Amurensis Cortex decoction pieces.So the process degree of samples is different

the process degree should be controlled according to the laws.