Objective: To develop a UPLC method for the determination of linarin luteolin

acacia-7-O-β-D-glucopyranoside and acacetin contents in different parts (stem

leaf

flower and fruit) of Buddleja lindleyana

and compare the distribution of 4 kinds of flavonoids in different parts of B. lindleyana. Method: The optimal conditions of separation and detection were achieved on an Acquity BEH C18 column (2.1 mm×50 mm

1.7 μm)

with acetonitrile (A)-0.1%formic acid solution (B) as the mobile phase for gradient elution

at a flow rate of 0.15 mL·min-1

with a detection wavelength of 330 nm. The column temperature was 30℃ and sample volume was 2 μL. Result: Linarin

luteolin

acacia-7-O-β-D-glucopyranosideand acacetin showed a good linearity in the range of 0.002 0-0.600 3 (r=0.999 7)

0.000 4-0.040 1(r=0.999 9)

0.000 4-0.039 9(r=0.999 9) and 0.000 2-0.020 0 μg (r=0.999 9) respectively. Their average recoveries were 100.2%

97.1%

94.3% and 92.4%. Conclusion: The method was accurate and simple with high repeatability and stability

and can be used for the determination of multiple flavonoids in different parts of B. lindleyana.