Objective: To study the effect of Lonicerae Japonicae Flos and Forsythiae Fructus on immune pathway of H1N1 Influenza A based on bioinformatics approach. Method: Target proteins of Lonicerae Japonicae Flos and Forsythiae Fructus

and genes of H1N1 Influenza A were searched in TCM Database @Taiwan

PubChem and Gene online public platform databases. Bioinformatics software was used to analyze the data sets

and explain the effect of Lonicerae Japonicae Flos and Forsythiae Fructus on immune pathway of H1N1 Influenza A. Result: There were 16 target proteins of Lonicerae Japonicae Flos

eight target proteins of Forsythiae Fructus and 32 genes of H1N1 influenza A. According to the bioinformatics analysis

Lonicerae Japonicae Flos and Forsythiae Fructus have their specific immune pathways

respectively. After the production pathway of IL-12 signaling and the pathway IL-8 signaling were treated with the drug pairs

the -log P value representing the correlation between drug target protein and biological pathway was higher than that of single administration of Lonicerae Japonicae Flos or Forsythiae Fructus.Matrix metalloproteinases(MMPs) involved in the most of specific immune pathways of Lonicerae Japonicae Flos

and PRKCA involved in the most of specific immune pathways of Forsythiae Fructus. Conclusion: Lonicerae Japonicae Flos and Forsythiae Fructus have their own characteristics in regulating specific immune pathways related to H1N1 Influenza A. When being used as a drug pair

they have more advantages in regulating the production pathway of IL-12 signaling and the pathway IL-8 signaling. MMPs and PRKCA may be the key proteins of Lonicerae Japonicae Flos and Forsythiae Fructus in regulating immune pathways.