Objective: To analyze and identify the alkaloids chemical constituents from the Menispermi Rhizoma by UPLC-Q-TOF-MS/MS. Method: Welch C18 reverse phase column (2.1 mm×100 mm

1.7μm) was adopted

with 0.1% ammonia solution (A)-acetonitrile (B) as the mobile phase for gradient elution.The flow rate was 0.3 mL·min-1; column temperature was 40 ℃; and the injection volume was 2 μL. ESI ion source was used as mass spectra

and the data were collected in the positive mode.The mass scanning range was between m/z 50-1 250.By using the target compound screening method in the mass spectrometry analysis software

the chemical components were identified according to retention time

accurate relative molecular mass and second mass spectrum fragmentation. Result: Under the optimized LC-MS condition

37 alkaloids compounds were identified based on the database of Scifinder

control substances and related references

including bis-benzyl isoquinoline compounds

apomorphine and isooxyphene oxide compounds

and monobenzylisoquinolines

et al. Conclusion: Based on UPLC-Q-TOF-MS/MS technology

relative molecular mass and fragmentation data determined by high performance liquid chromatography

as well as literature analysis

could rapidly and accurately identify the chemical components of Menispermi Rhizoma. This study could provide powerful evidences for further studying the pharmacodynamics material basis of Menispermi Rhizoma.