Objective: To discuss the effect of Qingzao Jiufei Tang on proliferation

invasion and metastasis-related proteins nuclear transcription factor-kappa B (NF-κB)

vascular endothelial growth factor (VEGF)

vascular endothelial growth factor receptor-1 (VEGFR-1)

matrix metalloprotein-9 (MMP-9) expression in CT26 mice colon cancer cells. Method: A total of 50 male BALB/c mice were randomly divided into model group

chemotherapy group [50 mg · kg-1 · (2d)-1]

high

medium and low-dose groups (15.2

7.6

3.8 g · kg-1 · d-1)

with 10 mice in each group. The colon cancer model was established by right axillary injection with CT26 cells. Qingzao Jiufei Tang group was given the corresponding dose of drug two weeks before modeling

5-FU group [50 mg · kg-1 · (2 d)-1] and model group (same volume of saline) started injection after modeling. Two weeks later

all of the mice were put to death

and tumor tissues were collected and weighed. We detected the expressions of NF-κB

VEGF

VEGFR-1

MMP-9 by the Western blot method. Result: The tumor weights of the high-dose group and the medium-dose group were decreased

compared with model group (P<0.01). The tumor inhibition rates of the chemotherapy group and high

middle and low-dose groups were 83.90%

60.98%

44.39%

21.46%. Compared with model group

the protein expressions of NF-κB and VEGF were significantly reduced

with significant differences between them (P<0.05

P<0.01). Compared with model group

the protein expressions of VEGFR-1 and MMP-9 were significantly lower in high

medium and low-dose groups

with significant differences between them (P<0.05

P<0.01). Conclusion: Qingzao Jiufei Tang can reduce the protein expressions of NF-κB

VEGF

VEGFR-1

MMP-9

and inhibit the invasion and metastasis of colon cancer cells in CT26 mice.