蓝布正提取物对血管性痴呆大鼠学习记忆能力及海马NT-3,BDNF蛋白表达的影响

刘明; 刘杨; 邓颖; 江蓓; 吕金田; 蔡兵

doi:10.13422/j.cnki.syfjx.2017170154

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蓝布正提取物对血管性痴呆大鼠学习记忆能力及海马NT-3,BDNF蛋白表达的影响

药理 | 更新时间：2024-01-04

- 蓝布正提取物对血管性痴呆大鼠学习记忆能力及海马NT-3,BDNF蛋白表达的影响
- Effect of Gei Herba Extract on Learning and Memory Ability and Expression of NT-3 and BDNF Protein in Hippocampus of Vascular Dementia Rats
- 中国实验方剂学杂志 2017年23卷第17期页码：154-158
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省科技合作计划项目（黔科合LH字[2015]7274号）;贵州省科技计划项目（黔科合院士站[2014]4013号）;贵州省协同创新中心建设项目（黔教合协同创新字[2013]02号）;贵州省普通高等学校工程研究中心建设项目（黔教合KY字[2014]22号）
- DOI：10.13422/j.cnki.syfjx.2017170154
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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刘明, 刘杨, 邓颖, 等. 蓝布正提取物对血管性痴呆大鼠学习记忆能力及海马NT-3,BDNF蛋白表达的影响[J]. 中国实验方剂学杂志, 2017,23(17):154-158.

LIU Ming, LIU Yang, DENG Ying, et al. Effect of Gei Herba Extract on Learning and Memory Ability and Expression of NT-3 and BDNF Protein in Hippocampus of Vascular Dementia Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 154-158.
刘明, 刘杨, 邓颖, 等. 蓝布正提取物对血管性痴呆大鼠学习记忆能力及海马NT-3,BDNF蛋白表达的影响[J]. 中国实验方剂学杂志, 2017,23(17):154-158. DOI： 10.13422/j.cnki.syfjx.2017170154.

LIU Ming, LIU Yang, DENG Ying, et al. Effect of Gei Herba Extract on Learning and Memory Ability and Expression of NT-3 and BDNF Protein in Hippocampus of Vascular Dementia Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(17): 154-158. DOI： 10.13422/j.cnki.syfjx.2017170154.

摘要

目的：观察蓝布正提取物（GHE）对血管性痴呆（VD）大鼠学习记忆能力的影响并探讨其作用的机制。方法：采用双侧颈总动脉结扎法制作VD大鼠模型，将大鼠随机分成假手术组，模型组，GHE高、中、低剂量组（7，3.5，1.75 g ·kg-1）和阳性药组（天保宁银杏叶片，0.007 g ·kg-1），各组灌胃相应药物，每日1次，连续35 d，在第30~35天进行Morris水迷宫实验检测大鼠的学习记忆能力，Morris水迷宫实验结束后处死大鼠，收集大脑样本，免疫组化法检测海马CA1区B细胞淋巴瘤-2（Bcl-2）相关X蛋白（Bax），Bcl-2，神经营养因子-3（NT-3），脑源性神经营养因子（BDNF）蛋白表达。结果：与模型组比较，GHE高、中剂量组可明显缩短大鼠的逃避潜伏期，增加穿越平台数（P<0.05）；GHE高、中、低剂量组可显著降低海马CA1区Bax表达，升高Bcl-2，NT-3，BDNF表达（P<0.05，P<0.01）。结论：GHE能改善VD大鼠的学习记忆能力，其机制可能与促进海马CA1区NT-3，BDNF的表达，抑制神经细胞凋亡有关。

Abstract

Objective: To explore the protective effect and mechanism of Gei Herba extract (GHE) on memory and learning ability of rats model of vascular dementia (VD). Method: Rats with vascular dementia were prepared with bilateral carotid artery ligation. Then the rats were randomly divided into 6 groups

namely sham operation group

model group

GHE high

medium and low dose groups (7

3.5

1.75 g · kg-1)

and Taponing positive control group (0.007 g · kg-1). The corresponding drug was given by oral administration for 35 d

once a day. The behavior tests were carried out by water maze task on day 30-35 to test the memory and learning abilities of the rats

then they were sacrificed at the end of water maze test. The protein expression levels of B-cell lymphoma-2(Bcl-2) assolated X protein(Bax)

Bcl-2

nerve neurotrophic factor-3(NT-3) and nerve neurotrophic factor-3(BDNF) in hippocampal CA1 region of brain samples collected after the last administration were detected by immunocytochemistry. Result: GHE high and middle dose groups can significantly shorten the escape latency and increase the number of cross platforms (P<0.05); GHE high

middle and low dose groups can significantly decline the expression levels of Bax and rise the expression levels of Bcl-2

NT-3 and BDNF (P<0.05

P<0.01). Conclusion: GHE can improve learning and memory abilities of VD rats

and its mechanism may be related to the promotion of NT-3 and BDNF expression in hippocampal CA1 region and the inhibition of neurons apoptosis.

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