Objective: To observe the intervention effect and mechanism of Yifei Jiedu decoction on the invasion and metastasis of lung cancer adenocarcinoma A549 cells. Method: A549 cells were cultured and treated with 150 μmol · L-1 CoCl2

150 μmol · L-1 CoCl2+15 g · L-1 Yifei Jiedu decoction

and 15 g · L-1 Yifei Jiedu decoction

respectively. Untreated cells were used for normal control. Morphological changes were observed under inverted microscope. Wound scratch assay were performed to further identify if Yifei Jiedu decoction could reduce the migration ability of A549 cells. Transwell was used to explore the intervention effect of Yifei Jiedu decoction on the invasion and movement of A549 cells. Expressions of E-cadherin

Vimentin

Snail

β-catenin and p-gsk-3β were examined by Western blot. Result: CoCl2 can induce morphological changes in A549 cells

which were accompanied by up-regulation of E-cadherin expression and down-regulation of Vimentin. Wound scratch assay indicated that Yifei Jiedu decoction could reduce the migration ability of A549 cells. Transwell assay showed that Yifei Jiedu decoction could inhibit the invasion of A549 cells(P<0.05). Western blot showed that Yifei Jiedu decoction could up-regulate the expressions of E-cadherin

p-gsk-3β protein and down-regulate the expressions of Vimentin

Snail and β-catenin protein respectively(P<0.05). Conclusion: Yifei Jiedu decoction could inhibit the migration and invasion of human adenocarinoma A549 cells via regulation of β-catenin expression.