Objective: To establish an HPLC fingerprint of water decoction of Moslae Herba for quality evaluation

and lay the foundation for the quality control of Xinjia Xiangruyin granules. Method: The chromatographic fingerprints of 10 batches of water decoction of Moslae Herba from different places of origin were obtained on Phenomenex Luna C18 (4.6 mm×250 mm

5 μm)

with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The column temperature was maintained at 40℃ at a flow rate of 1 mL·min-1

and the detection wavelength was 269 nm. 10 batches of fingerprints were imported into Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012.130723) for chromatographic peak matching. With caffeic acid as the reference peak

the midpoint method was used to establish the reference fingerprint

which was then compared with the fingerprint of 10 batches of Moslae Herba. Result: The HPLC fingerprint of Moslae Herba showed 14 common peaks. The similarities of 10 peaks from different origins were all more than 0.90.Then the analysis results on relative peak areas

showed less differences in the quality of Moslae Herba from 10 origins. Conclusion: With good reproducibility and feasibility

this method can be used as the means to assess the quality of Moslae Herba and provides reference for preparing the quality standard of Xinjia Xiangruyin granules.