Objective:To analyze the saponins in Yiqi Fumai freeze-dried powder for injection by HPLC-Q-TOF-MS

and provide reference for quantitative study of saponins in the preparation and its pharmacodynamical material basis. Method:The fragmentation patterns of ginsenoside Rg1

Re and Rb1 were analyzed in detail

and the saponins in Yiqi Fumai freeze-dried powder for injection were identified by HPLC-Q-TOF-MS. Result:There were two main pathways for mass spectrometric cleavage of ginsenoside Rg1

Re and Rb1:①loss of all aglycones until forming a stable deprotonated parent nucleus

②aglycones fragmentation and the formation of characteristic fragment ions.On the basis of the fragmentation law of mass spectrometry

21 saponins in Yiqi Fumai freeze-dried powder for injection were identified

in addition to ginsenoside Rg1

Rb1

Re

Rc

Rd

Rf

Rg3 and notoginsenoside R1

the other 13 saponins were first reported in this preparation.At the same time

deoxyschizandrin was initially identified by literature comparison. Conclusion:Most of the saponins with high content in Yiqi Fumai freeze-dried powder for injection are identified by HPLC-Q-TOF-MS

which can provide material foundation for further investigation of this preparation.