健脾消癌方抑制结肠癌增殖的作用机制

罗燕; 罗吉; 蒋益兰; 李勇敏; 谭小宁; 吕元; 马荣丽

doi:10.13422/j.cnki.syfjx.20180683

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健脾消癌方抑制结肠癌增殖的作用机制

健脾消癌方抑制结直肠癌转移分子机制研究专题 | 更新时间：2024-01-04

- 健脾消癌方抑制结肠癌增殖的作用机制
- Mechanism of Jianpi Xiaoai Prescription in Inhibiting Colon Cancer Proliferation
- 中国实验方剂学杂志 2018年24卷第6期页码：151-155
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81373602）;湖南省自然科学基金青年基金项目（2017JJ3186）;湖南省中医药研究院重点项目（201604，201504）
- DOI：10.13422/j.cnki.syfjx.20180683
  中图分类号： R285
- 纸质出版日期：2018
- 稿件说明：
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罗燕, 罗吉, 蒋益兰, 等. 健脾消癌方抑制结肠癌增殖的作用机制[J]. 中国实验方剂学杂志, 2018,24(6):151-155.

LUO Yan, LUO Ji, JIANG Yi-lan, et al. Mechanism of Jianpi Xiaoai Prescription in Inhibiting Colon Cancer Proliferation[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 151-155.
罗燕, 罗吉, 蒋益兰, 等. 健脾消癌方抑制结肠癌增殖的作用机制[J]. 中国实验方剂学杂志, 2018,24(6):151-155. DOI： 10.13422/j.cnki.syfjx.20180683.

LUO Yan, LUO Ji, JIANG Yi-lan, et al. Mechanism of Jianpi Xiaoai Prescription in Inhibiting Colon Cancer Proliferation[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 151-155. DOI： 10.13422/j.cnki.syfjx.20180683.

摘要

目的：观察健脾消癌方对结肠癌HCT116细胞中微小RNA-21（microRNA-21，miR-21），第10号染色体丢失的磷酸酶基因（phasphatase and tensin homo-log deleted in chromosome 10，PTEN）表达的影响，探讨健脾消癌方抑制结肠癌转移的可能作用机制。方法： 10%，15%，20%健脾消癌方含药血清处理HCT116细胞后，实时荧光定量PCR （Real-time PCR）检测miR-21，PTEN mRNA的表达水平，蛋白免疫印迹法（Western blot）检测PTEN蛋白的表达水平。结果：与空白组比较，健脾消癌方低剂量组miR-21 mRNA相对表达量降低，PTEN mRNA相对表达量升高，但差异无统计学意义；健脾消癌方中剂量组miR-21 mRNA相对表达明显降低，PTEN mRNA相对表达明显升高（P<0.05）；健脾消癌方高剂量组miR-21 mRNA相对表达量显著降低，PTEN mRNA相对表达量显著升高（P<0.01）；与健脾消癌方中、低剂量组比较，健脾消癌方高剂量组miR-21 mRNA相对表达量明显降低，PTEN mRNA相对表达量明显升高（P<0.05）。与空白组比较，健脾消癌方中、低剂量组PTEN蛋白相对表达量升高，差异无统计学意义；与空白组比较，健脾消癌方高剂量组PTEN蛋白相对表达量明显上升（P<0.05）；与健脾消癌方中、低剂量组比较，健脾消癌方高剂量组PTEN蛋白相对表达量明显升高（P<0.05）。结论：健脾消癌方可能通过降低miR-21的表达，上调miR-21靶基因PTEN蛋白表达抑制结肠癌的增殖。

Abstract

Objective: To observe the effect of Jianpi Xiaoai prescription on the expressions of microRNA-21 (miR-21)

phasphatase and tensin homo-log deleted in chromosome 10 (PTEN) in colon cancer HCT116 cells

in order to investigate the possible mechanism of Jianpi Xiaoai prescription on the metastasis of colon cancer.Method: After the treatment with drug serum (10%

15% and 20%)

Real-time PCR was used to detect the mRNA expressions of miR-21

PTEN

and the protein expression of PTEN was detected by Western blot.Result: Compared with control group

the relative mRNA expression of miR-21 was decreased

while the relative mRNA expression of PTEN was significantly increased in the low-dose Jianpi Xiaoai prescription group. Compared with control group

the relative mRNA expression of miR-21 in high-dose group was statistically lower

while the mRNA relative expression of PTEN in high-dose group was higher (P<0.05). Compared with the low and middle-dose groups

the relative mRNA expression of miR-21 in high-dose group was decreased

while the relative mRNA expression of PTEN was significantly increased (P<0.05). Compared with control group

the relative protein expression of PTEN in high-dose group was significantly higher than that in low-dose group (P<0.05). The relative protein expression of PTEN in high-dose group was higher than that in low-dose group and middle-dose groups (P<0.05).Conclusion: Jianpi Xiaoai prescription can inhibit the proliferation of colon cancer by reducing the expression of miR-21 and improving the expression of anti-cancer protein PTEN.

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