健脾消癌方对大肠癌肝转移裸鼠模型肝组织PI3K/Akt通路相关蛋白表达的影响

王容容; 谭小宁; 李勇敏; 唐蔚; 王其美; 杨晓; 简小兰; 蒋益兰

doi:10.13422/j.cnki.syfjx.20180688

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健脾消癌方对大肠癌肝转移裸鼠模型肝组织PI3K/Akt通路相关蛋白表达的影响

健脾消癌方抑制结直肠癌转移分子机制研究专题 | 更新时间：2024-01-04

- 健脾消癌方对大肠癌肝转移裸鼠模型肝组织PI3K/Akt通路相关蛋白表达的影响
- Effect of Jianpi Xiaoai Prescription on Expressions of Protein Related to PI3K/Akt Pathway in Liver Tissues of Nude Mice Model of Colorectal Cancer with Liver Metastasis
- 中国实验方剂学杂志 2018年24卷第6期页码：177-181
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81373602，81774287）;中央引导地方科技发展专项（2017CT5029）;湖南中医药大学在读研究生创新项目（2016CX06）
- DOI：10.13422/j.cnki.syfjx.20180688
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
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王容容, 谭小宁, 李勇敏, 等. 健脾消癌方对大肠癌肝转移裸鼠模型肝组织PI3K/Akt通路相关蛋白表达的影响[J]. 中国实验方剂学杂志, 2018,24(6):177-181.

WANG Rong-rong, TAN Xiao-ning, LI Yong-min, et al. Effect of Jianpi Xiaoai Prescription on Expressions of Protein Related to PI3K/Akt Pathway in Liver Tissues of Nude Mice Model of Colorectal Cancer with Liver Metastasis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 177-181.
王容容, 谭小宁, 李勇敏, 等. 健脾消癌方对大肠癌肝转移裸鼠模型肝组织PI3K/Akt通路相关蛋白表达的影响[J]. 中国实验方剂学杂志, 2018,24(6):177-181. DOI： 10.13422/j.cnki.syfjx.20180688.

WANG Rong-rong, TAN Xiao-ning, LI Yong-min, et al. Effect of Jianpi Xiaoai Prescription on Expressions of Protein Related to PI3K/Akt Pathway in Liver Tissues of Nude Mice Model of Colorectal Cancer with Liver Metastasis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 177-181. DOI： 10.13422/j.cnki.syfjx.20180688.

摘要

目的：研究健脾消癌方对大肠癌肝转移裸鼠模型肝组织中磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B （Akt）通路相关蛋白表达的影响，探讨其拮抗大肠癌肝转移的作用机制。方法：随机抽取10只BALB/C-nu裸鼠作为空白组，其余裸鼠建立人大肠癌细胞肝转移裸鼠模型，建模3 d后取存活裸鼠30只，随机分为模型组，健脾消癌方组（41.6 g · kg-1），人参皂苷Rg3组（5.2 mg · kg-1），各组裸鼠灌胃相应药物及生理盐水4周后脱颈处死，观察各组裸鼠肝转移情况，采用蛋白免疫印迹法（Western blot）测定肝组织第10号染色体同源缺失性磷酸酶和张力蛋白基因（PTEN），磷酸化第10号染色体同源缺失性磷酸酶和张力蛋白基因（p-PTEN），Akt，磷酸化蛋白激酶（p-Akt）蛋白表达情况。结果：健脾消癌方及人参皂苷Rg3组肝转移率明显低于模型组（P<0.05）；与空白组比较，模型组中p-PTEN，p-Akt蛋白表达明显升高（P<0.05），与模型组比较，健脾消癌方组与人参皂苷Rg3组肝组织中p-PTEN，p-Akt蛋白表达明显降低（P<0.05）；健脾消癌方组与人参皂苷Rg3组p-PTEN，p-Akt表达差异无统计学意义。与空白组比较，模型组PTEN，Akt蛋白表达明显降低（P<0.05）；与模型组比较，健脾消癌方组与人参皂苷Rg3组PTEN，Akt蛋白表达量明显升高（P<0.05）；健脾消癌方组与人参皂苷Rg3组PTEN，Akt蛋白表达差异无统计学意义。结论：健脾消癌方可拮抗大肠癌肝转移，其作用机制可能与升高PTEN蛋白表达，降低p-PTEN，p-Akt蛋白表达有关。

Abstract

Objective: To investigate the effect of Jianpi Xiaoai prescription on the expressions of proteins related to phosphatidyl inositol 3-kinase/protein kinase B (PI3K/Akt) signal pathway in liver tissues of nude mice model of colorectal cancer with liver metastasis

in order to explore the mechanism in antagonizing the liver metastasis of colorectal cancer.Method: Ten BALB/C-nu nude mice were randomly selected as the control group. The remaining mice were used to establish the model of colon cancer with liver metastasis. Three days after modeling

30 survived nude mice were collected and divided into model group

Jianpi Xiaoai prescription group (41.6 g · kg-1) and Rg3 group (5.2 mg · kg-1). After the mice were given the corresponding drugs and physiological saline by gavage for 4 weeks

the protein expressions of chromosome 10 homologous phosphatase and tension protein gene (PTEN)

phosphorylation chromosome 10 homologous phosphatase and tension protein gene (p-PTEN)

Akt

phosphorylation protein kinase B (p-Akt) in liver tissues were evaluated by Western blot.Result: The liver metastasis rate and the expressions of p-PTEN

p-Akt in liver tissues of Jianpi Xiaoai prescription group and Rg3 group were significantly lower than those in model group (P<0.05). The expressions of p-PTEN

p-Akt in liver tissues were significantly higher than those of control group (P<0.05); but there was no significant difference between Jianpi Xiaoai prescription group and positive control group. The expression of PTEN

Akt in model group were significantly lower than control group(P<0.05). The expressions of PTEN

Akt in Jianpi Xiaoai prescription group and positive control group were significantly higher than those in model group (P<0.05)

there was no significant difference between Jianpi Xiaoai prescription group and Rg3 group.Conclusion: Jianpi Xiaoai prescription could reduce the liver metastasis rate of colorectal cancer

the mechanism may increase the expression of PTEN in liver tissues

and down-regulate the expressions of p-PTEN

p-Akt.

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