Objective: To explore the effects of Qinyu Sanlong decoction on expression of secreted frizzled-related proteins (SFRP)-2 antagonistic Wnt signal pathway. Method: Based on the previous research

LLC cells were used to construct lung cancer model. The mice bearing tumor were divided into model group (M)

Qinyu Sanlong decoction groups (low

middle and high dose groups

named as QL

QM

QH respectively)

chemotherapy group (C)

and combination group (CQH). Qinyu Sanlong decoction groups were administrated with 20.12

40.24

and 80.48 g · kg-1 · d-1 by gavage. C group was given with 0.4 mL cisplatin by intraperitoneal injecting. CQH group was administered with high dosage of Qinyu Sanlong decoction by gavage and cisplatin by intraperitoneal injecting. M group was administered with the same amount of 0.9% sodium chloride solution

once a day for 21 d. The tumors were weighed to calculate the tumor inhibition rate. Transmission electron microscopy was used to observe the ultrastructure of tumor. The SFRP-2 and β-catenin protein expression levels in tumor tissues were detected by Western blot. Result: As compared with M group

every medication group could down-regulate β-catenin expression (P<0.01); as compared with QL group

other medication groups also could down-regulate β-catenin expression (P<0.01); as compared with C group

the down-regulation was more obvious in QM group

QH group and CQH group (P<0.05

P<0.01). The results of SFRP-2 showed that SFRP-2 expression was up-regulated in QM and QH groups as compared with M group (P<0.05

P<0.01); every other medication group also could up-regulate SFRP-2 expression as compared with QL group (P<0.01); QM and QH groups could up-regulate SFRP-2 expression as compared with C group (P<0.05

P<0.01). Also

apoptosis was found under electron microscope in medication groups

especially in QH

C and CQH groups. Conclusion: Qinyu Sanlong decoction can effectively inhibit tumor growth

induce apoptosis

promote the SFRP-2 expression and inhibit the Wnt/β-catenin signal pathway.