Objective: To identify the chromatographic components of 60% methanol extract in Cistanches Herba cultured in Tarim desert by HPLC-MS.Method: HPLC-MS analysis was performed on a Agilent ZORBAX Eclipse Plus C18 column(4.6 mm×250 mm

5 μm).The mobile phase was acetonitrile-0.1% formic acid aqueous solution by gradient elution at the flow rate of 1 mL·min-1

and the detection wavelength was 254 nm. The column temperature was set at 25℃

and the injection volume was 10 μL.Result: Sixteen chromatographic peaks/seventeen components were accurately identified by comparing the retention time

molecular weights and fragment ions of the compounds we isolated and identified. These chromatographic components were determined as coniferin

demethyl syringin

syringin

echinacoside

cistanoside A

verbascoside (tubuloside A)

cistanoside B

isoverbascoside

2'-acetylcistanoside A

cistanoside C

isocistanoside C

2'-acetylverbascoside

tubuloside B

epimeridinoside A

cistanoside K and cistanoside J.Conclusion: The HPLC-MS method is suitable for the identification of the chromatographic components of 60% methanol extract in Cistanches Herba. This study will provide the scientific evidence for comprehensively analyzing the chemical constituents of this medicinal herb.