不同果形栀子鲜品不同极性部位HPLC指纹图谱

梁献葵; 王艳慧; 雷敬卫; 龚海燕; 唐维维; 谢彩侠

doi:10.13422/j.cnki.syfjx.20182317

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不同果形栀子鲜品不同极性部位HPLC指纹图谱

质量评价 | 更新时间：2021-02-09

- 不同果形栀子鲜品不同极性部位HPLC指纹图谱
- HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes
- 中国实验方剂学杂志 2019年25卷第2期页码：186-195
- 作者机构：
  
  1.河南中医药大学，郑州　 450046；
  2.仲景宛西制药股份有限公司，郑州　 450046
- 作者简介：
  
  梁献葵，在读硕士，从事中药质量分析研究，E-mail: 646909449@qq.com
  *雷敬卫，博士，硕士生导师，从事中药质量分析研究，Tel：0371-656955281，E-mail：yjs2017tg@126.com
- 基金信息：
  
  河南省中药材开发工程技术研究中心项目(142102313109);河南省科技科技攻关项目(162102310448)
- DOI：10.13422/j.cnki.syfjx.20182317
  中图分类号： R284.1; R289; R22; R2-031
- 收稿日期：2018-04-08，
  
  网络出版日期：2018-09-15，
  
  纸质出版日期：2019-01-20
- 稿件说明：
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梁献葵, 王艳慧, 雷敬卫, 等. 不同果形栀子鲜品不同极性部位HPLC指纹图谱[J]. 中国实验方剂学杂志, 2019,25(2):186-195.

Xian-kui LIANG, Yan-hui WANG, Jing-wei LEI, et al. HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 186-195.
梁献葵, 王艳慧, 雷敬卫, 等. 不同果形栀子鲜品不同极性部位HPLC指纹图谱[J]. 中国实验方剂学杂志, 2019,25(2):186-195. DOI： 10.13422/j.cnki.syfjx.20182317.

Xian-kui LIANG, Yan-hui WANG, Jing-wei LEI, et al. HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 186-195. DOI： 10.13422/j.cnki.syfjx.20182317.

摘要

目的：

研究并比较不同果形栀子鲜品不同极性部位(石油醚部位、三氯甲烷部位、乙酸乙酯部位、正丁醇部位、水部位)指纹图谱，深入了解其化学成分含量差异及分布情况。

方法：

将栀子用水回流提取，得水提取物，取水提物0.1 g，用水50 mL溶解后，依次用石油醚、三氯甲烷、乙酸乙酯、正丁醇进行萃取，得到各萃取相及萃取后的水相，浓缩至浸膏。建立HPLC指纹图谱，并采用相似度评价系统软件进行数据分析。

结果：

不同产地栀子水提物三氯甲烷部位指纹图谱可以将福建、河南、江西产栀子分开。福建栀子水提物与河南、江西的区别主要体现在石油醚部位，且其脂溶性成分比河南、江西栀子多；河南栀子水提物与福建、江西栀子的区别主要体现在乙酸乙酯部位，且其环烯迷萜苷类成分含量明显高于福建、江西栀子。江西栀子水提物与河南、福建栀子的差异主要体现在正丁醇部位，其无有机酸类峰C1。六、七棱栀子：栀子水提物的三氯甲烷部位指纹图谱可以将福建与河南的六、七棱栀子区分开，且七棱栀子各成分含量均大于六棱；栀子水提物的石油醚和乙酸乙酯部位指纹图谱均可以将江西六、七棱栀子区分开。河南六棱栀子Z3峰明显高于七棱；江西七棱栀子水提物三氯甲烷、乙酸乙酯部位各成分含量大于六棱。

结论：

福建、河南、江西栀子化学成分含量及种类存在显著差异；六、七棱栀子图谱差异主要体现在峰高。

Abstract

Objective:

To investigate and compare the fingerprints of different polarity fractions (petroleumether

chloroform

ethyl acetate

-butanol

water) of fresh Gardeniae Fructus with different fruit shapes

and further understand the content difference and distribution of its chemical composition.

Method:

Gardeniae Fructus was reflux extracted by water in order to obtain the water extract; water extract 0.1 g and dissolved with 50 mL water

then it was extracted by petroleum ether

chloroform

ethyl acetate and

-butanol in turn in order to obtain the different extraction phases and the water phase. Each phase was condensed to extractum. Finally

the samples were analyzed by high performance liquid chromatography (HPLC) fingerprints and the similarity evaluation software was used for data analysis.

Result:

Fingerprint of chloroform fraction of water extract in gardenia from different habitats can be used to distinguish Gardeniae Fructus from Fujian

Henan and Jiangxi. The differences between the water extract of Gardeniae Fructus from Fujian and those of Henan and Jiangxi were mainly manifested in the petroleum ether fraction

and the fat-soluble components of Gardeniae Fructus were more than those of Henan and Jiangxi. The differences between the water extract of Gardeniae Fructus from Henan and those of Fujian and Jiangxi were mainly manifested in the ethyl acetate fraction

and the content of iridoid glycosides was significantly higher than that in Fujian and Henan. The differences between the water extract of gardenia from Jiangxi and those of Fujian and Henan were mainly manifested in the

-butanol fraction

organic acid peak C1 not detected. The fingerprint of chloroform fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Fujian and Henan

and the contents of all components of Gardeniae Fructus of seven ribs were more than those in Gardeniae Fructus of six ribs. The fingerprint of petroleum ether fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Jiangxi. The Z3 peak of Gardeniae Fructus of six ribs from Henan was obviously higher than that of Gardeniae Fructus of seven ribs. The contents of all components on chloroform and ethyl acetate fractions of water extract in Gardeniae Fructus of seven ribs were significantly higher than those of Gardeniae Fructus of six ribs.

Conclusion:

There are significant differences on chemical constituents and content among Gardeniae Fructus from Fujian

Henan and Jiangxi. The main difference of fingerprint between Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs is the peak height.

关键词

Keywords

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