基于线粒体融合-裂解探讨化瘀祛痰方对动脉粥样硬化家兔心肌纤维化的抑制作用

郑曲; 贾连群; 宋囡; 陈宁; 陈丝; 杨关林

doi:10.13422/j.cnki.syfjx.20200340

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基于线粒体融合-裂解探讨化瘀祛痰方对动脉粥样硬化家兔心肌纤维化的抑制作用

药理 | 更新时间：2021-02-09

- 基于线粒体融合-裂解探讨化瘀祛痰方对动脉粥样硬化家兔心肌纤维化的抑制作用
- Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis
- 中国实验方剂学杂志 2020年26卷第3期页码：71-77
- 作者机构：
  
  辽宁中医药大学，沈阳　 110032
- 作者简介：
  
  [第一作者]　郑曲，博士，主治医师，从事化痰祛瘀方在冠脉粥样硬化中的作用及机制研究，E-mail：362523993@qq.com
  *杨关林，博士，教授，博士后导师，从事中医脾脏象理论研究，E-mail：yang_guanlin@163.com
- 基金信息：
  
  国家自然科学基金项目(81774022);国家重点基础研究发展计划（973计划）项目(2013CB531704)
- DOI：10.13422/j.cnki.syfjx.20200340
  中图分类号： R22; R24; R289; R285
- 收稿日期：2019-05-18，
  
  网络出版日期：2019-10-18，
  
  纸质出版日期：2020-02-05
- 稿件说明：
移动端阅览
郑曲, 贾连群, 宋囡, 等. 基于线粒体融合-裂解探讨化瘀祛痰方对动脉粥样硬化家兔心肌纤维化的抑制作用[J]. 中国实验方剂学杂志, 2020,26(3):71-77.

Qu ZHENG, Lian-qun JIA, Nan SONG, et al. Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(3): 71-77.
郑曲, 贾连群, 宋囡, 等. 基于线粒体融合-裂解探讨化瘀祛痰方对动脉粥样硬化家兔心肌纤维化的抑制作用[J]. 中国实验方剂学杂志, 2020,26(3):71-77. DOI： 10.13422/j.cnki.syfjx.20200340.

Qu ZHENG, Lian-qun JIA, Nan SONG, et al. Study on Inhibitory Effect of Huayu Qutan Recipe on Myocardial Fibrosis in Atherosclerotic Rabbits Based on Mitochondrial Fusion-lysis[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(3): 71-77. DOI： 10.13422/j.cnki.syfjx.20200340.

摘要

目的：

探讨化瘀祛痰方通过影响线粒体融合-裂解对动脉粥样硬化家兔心肌纤维化的抑制作用。

方法：

选取SPF级健康雄性家兔36只，随机选取6只为正常组，给予普通颗粒饲料；另30只采用高脂饲料建立动脉粥样硬化模型。模型复制成功后，随机分为模型组、化瘀祛痰方低、中、高剂量组(4.0，8.0，16.0 g·kg

-1

)及辛伐他汀组(1.4 mg·kg

-1

)，各6只。各给药组按剂量给予相应药物，连续给药4周。全自动生物化学分析仪检测各组家兔血清血脂水平，马松(Masson)染色法观察各组家兔心肌纤维化程度，免疫组化法检测心肌组织线粒体融合蛋白1(Mitofusin1)，线粒体融合蛋白2(Mitofusin2)，视神经萎缩蛋白1(Opa1)，发动蛋白相关蛋白1(Drp1)，分裂蛋白1(Fis1)表达水平。

结果：

与正常组比较，模型组家兔血清总胆固醇(TC)，甘油三酯(TG)，低密度脂蛋白胆固醇(LDL-C)水平明显升高，高密度脂蛋白胆固醇(HDL-C)水平明显降低，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显降低，Drp1，Fis1表达水平明显升高(

0.05，

0.01)，与模型组比较，辛伐他汀组及化瘀祛痰方低、中、高剂量组家兔血清TC，TG，LDL-C水平明显降低，HDL-C水平明显升高，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显升高，Drp1，Fis1表达水平明显降低(

0.05，

0.01)，与化瘀祛痰方高剂量组比较，辛伐他汀组及化瘀祛痰方低、中剂量组血清TC，TG，LDL-C水平明显升高，HDL-C水平明显降低，心肌组织Mitofusin1，Mitofusin2，Opa1表达水平明显降低，Drp1，Fis1表达水平明显升高(

0.05，

0.01)。

结论：

化瘀祛痰方能有效调节血脂，抑制粥样硬化家兔心肌纤维化，且化瘀祛痰方剂量越高效果越明显，推测其作用可能与调节心肌细胞线粒体融合-裂解相关蛋白Mitofusin1，Mitofusin2及Opa1，Drp1，Fis1表达有关。

Abstract

Objective:

To analysis the effect of Huayu Qutan recipe on myocardial fibrosis in atherosclerotic rabbits based on mitochondrial fusion-lysis.

Method:

The 36 SPF healthy male rabbits were selected

and 6 rabbits were selected randomly as the normal group

and given normal pellet feed

another 30 rats were fed with high fat diet to establish atherosclerosis model.After successful replication of animal models

they were randomly divided into model group

Huayu Qutan recipe low-dose

medium-dose and high-dose group (4.0

8.0

16.0 g·kg

-1

) and simvastatin group (1.4 mg·kg

-1

)

6 rats each.Each group was given corresponding drugs according to the dosage

continuous administration for 4 weeks.The serum lipid levels in rabbits of each group were detectived by automatic biochemical analyzer

the degree of myocardial fibrosis was measured by Masson staining

and expression levels of mitochondrial fusion protein 1 (Mitofusin 1)

mitochondrial fusion protein 2 (Mitofusin 2)

optic atrophy protein 1 (Opa1)

promoter protein 1 (Drp1)

mitogen 1 (Fis1) in myocardial tissue were detected by immunohistochemistry.

Result:

Compared with normal group

the levels of total cholesterol (TC)

triglyceride (TG)

low density lipoprotein cholesterol (LDL-C) in model group were significantly increased

levels of density lipoprotein cholesterol (HDL-C) were significantly decreased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly decreased

the expression levels of Drp1 and Fis1 were significantly increased(

0.05

0.01)

compared with model group

the levels of serum TC

LDL-C in simvastatin group and Huayu Qutan recipe low-dose

medium and high-dose group were significantly decreased

levels of HDL-C were significantly increased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly increased

the expression levels of Drp1 and Fis1 were significantly decreased (

0.05

0.01)

compared with the Huayu Qutan recipe high-dose group

the levels of serum TC

LDL-C in simvastatin group and Huayu Qutan recipe low

medium-dose group were significantly increased

the expression levels of Mitofusin 1

Mitofusin 2 and Opa1 in myocardial tissue were significantly decreased

the expression levels of Drp1 and Fis1 were significantly increased(

0.05

0.01).

Conclusion:

Huayu Qutan recipe can effectively regulate blood lipid and inhibit myocardial fibrosis in atherosclerotic rabbits

and the higher the dose of Huayu Qutan recipe

the more obvious the effect is

it is speculated that its effect may be related to the regulation of the expression of mitochondrial fusion-lysis related proteins such as Mitofusin1

Mitofusin2 and Opa1

Drp1 and Fis1 in myocardial cells.

关键词

Keywords

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