Objective: To study the influence of cream processing on metabolism and tissue distribution of bitter almond in rat. Method :Amygdalin was set as control group given by mainline

bitter almond and its cream product were given to SD rats by oral

HPLC was employed for the determination of amygdalin and its metabolite in blood

heart

liver

spleen

lung and kidney

and LC-MSn was used for further identification of the metabolite structure. Result: Amygdalin was distributed in the tissues as prototype after given by mainline

while in the groups of oral administration

it was distributed as the secondary metabolite prunasin;and compared to bitter almond crude material

the time of prunasin distribution in the tissues was prolonged after cream processing

and showed two peaks phenomenon. Conclusion: Cream processing have a significant effect on the metabolism and tissue distribution of bitter almond

this provides information for the mechanism of bitter almond’s processing.