Objective: To observe the regulation effect of angelica sinensis polysaccharides on cytokines of human keratinocyte (HKC) and procollagen secretion of fibroblast cells (FC) in dermis

and to investigate the effect of angelica sinensis polysaccharides in skin wound healing. Method: HKC and FC were cultured in DMEM medium with 10% FBS in an incubator containing 5%CO2 at 37 ℃. And pretreated with extract of Angelica Sinensis polysaccharides

The proliferation of fibroblast cells was measured by monotetrazolium (MTT) colourmetric assay. The expression of transforming growth factor-beta (TGF-β) and interleukin-1 alpha(IL-lα) was evaluated by immunohistochemistry. The effect on fibroblast cells proliferation and collagen type Ⅲ excretion was observed. Result: Angelica sinensis polysaccharides could directly stimulate the HKC’s proliferation effect on FC. Results showed that HKC’s proliferation effect was increased with the concentration of angelica sinensis polysaccharides arising and the peak of HKC’s proliferation effect was obtained with 0.2 g·L-1. The angelica sinensis polysaccharides could regulate excretion of TGF-β and IL-lα in a dose-dependent manner

and proliferation of fibroblast and synthesis of collagen could be enhanced. Conclusion: Angelica sinensis polysaccharides can regulate the expression of TGF-β and IL-lα on keratinocyte and collagen type Ⅲ excretion

so as to stimulate proliferation of fibroblasts. It indicates that human keratinocyte and fibroblast are involved in wound healing. Angelica Sinensis polysaccharides plays an important role in wound healing process.