芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白的影响

王晓辉; 王伊林; 靳小石

doi:10.13422/j.cnki.syfjx.20181119

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芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白的影响

药理 | 更新时间：2024-01-04

- 芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白的影响
- Effect of Aloe-emodin on Growth, Migration and Fibrous Actin of Hepatoma HepG2 Cells
- 中国实验方剂学杂志 2018年24卷第11期页码：111-116
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西壮族自治区卫生和计划生育委员会科研课题项目（Z2016668）
- DOI：10.13422/j.cnki.syfjx.20181119
  中图分类号： R285.5
- 纸质出版：2018
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王晓辉, 王伊林, 靳小石. 芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白的影响[J]. 中国实验方剂学杂志, 2018,24(11):111-116.

WANG Xiao-hui, WANG Yi-lin, JIN Xiao-shi. Effect of Aloe-emodin on Growth, Migration and Fibrous Actin of Hepatoma HepG2 Cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 111-116.
王晓辉, 王伊林, 靳小石. 芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白的影响[J]. 中国实验方剂学杂志, 2018,24(11):111-116. DOI： 10.13422/j.cnki.syfjx.20181119.

WANG Xiao-hui, WANG Yi-lin, JIN Xiao-shi. Effect of Aloe-emodin on Growth, Migration and Fibrous Actin of Hepatoma HepG2 Cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 111-116. DOI： 10.13422/j.cnki.syfjx.20181119.

摘要

目的：探讨芦荟大黄素对肝癌HepG2细胞生长、迁移及纤维状肌动蛋白（F-actin）的影响。方法：通过对人正常肝细胞株QSG-7701细胞的药物毒性实验筛选出芦荟大黄素对人正常肝细胞无明显毒性的最大无毒浓度（TC0）；以TC0为最高浓度并稀释成3个不同的芦荟大黄素浓度，作用于体外培养人肝癌HepG2细胞为芦荟大黄素组，另设置未加药物HepG2细胞为空白组；通过噻唑蓝（MTT）比色法检测两组细胞作用48 h后细胞增殖抑制率；采用细胞小室移动实验及划痕实验检测两组细胞迁移能力；高内涵细胞成像系统分析芦荟大黄素对HepG2细胞F-actin表达的影响；蛋白免疫印迹法（Western blot）检测各组细胞磷酸化内皮型一氧化氮合酶（p-eNOS），核转录因子-κB p65（NF-κB p65）蛋白表达。结果：芦荟大黄素对人正常肝细胞株QSG-7701细胞的TC0为50 μmol·L-1，以TC0为最高浓度，将芦荟大黄素（10，30，50 μmol·L-1）作用于HepG2细胞；与空白组相比，芦荟大黄素组细胞培养48 h后增殖抑制率升高，且呈剂量递增型（P<0.01）；芦荟大黄素组细胞培养48 h后划痕距离增宽，迁移能力降低，且呈剂量递减型（P<0.01）；F-actin面积缩小，排列紊乱且不规则，边缘模糊，且呈浓度递减型（P<0.01）；芦荟大黄素组细胞NF-κB p65表达增多，p-eNOS表达减少，且呈浓度递减型（P<0.01）。结论：芦荟大黄素可能通过增强NF-κB p65，降低p-eNOS表达，促进F-actin解聚，减少微丝形成，从而降低肝癌HepG2细胞的增殖和迁移能力，发挥其抑制肿瘤的作用。

Abstract

Objective: To investigate the effect of aloe-emodin on the growth

migration and fibrous actin (F-actin) of hepatoma HepG2 cells. Method: The maximum toxic concentration (TC0) of aloe-emodin on human normal hepatocytes was screened out in the drug toxicity experiment for human normal hepatocyte cell line QSG-7701.TC0 was taken as the highest concentration

diluted to 3 different concentrations of aloe-emodin

and used to treat human hepatoma HepG2 cells in vitro for the aloe-emodin group; another untreated HepG2 cells were set up as a blank group; cell proliferation inhibition rate was measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay after 48 hours. Cell migration assay and scratch assay were used to detect the cell migration in both groups. The effect of aloe-emodin on the expression of HepG2 cell F-actin was analyzed by high-content cell imaging system. The expressions of phosphorylated endothelial nitric oxide synthase (p-eNOS) and nuclear transcription factor-κB p65 (NF-κB p65) in HepG2 cells were detected by Western blot. Result: TC0 of aloe-emodin on human normal hepatocyte cell line QSG-7701 was 50 μmol·L-1. TC0 was taken as the highest concentration to treat HepG2 cells with aloe-emodin (10

50 μmol·L-1). Compared with blank group

The proliferation inhibition rate of aloe-emodin group increased after 48 hours of culture

indicating the dose escalation thpe (P<0.01)

after 48 hours of culture

the scratch distance of aloe-emodin group was broadened

and the migrating ability of aloe-emodin group was decreased

indicating the dose decline type (P<0.01); F-actin area was shorten

disordered

irregular and fuzzy

indicating the dose decline type (P<0.01); the expression of NF-κB p65 in aloe-emodin group was increased

while the expression of p-eNOS was decreased

indicating the dose decline type (P<0.01). Conclusion: Aloe-emodin may reduce the proliferation and migration of HepG2 cells by increasing NF-κB p65

inhibiting the expression of p-eNOS

promoting the depolymerization of F-actin

and reducing the formation of microfilaments

with an effect in inhibiting tumor.

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