最新刊期
      卷 ， 期 5 ， 2003
      • QIN Chun-mei,LIU You-ping,YAN dan,CHEN Hong-ping
        Issue 5, Pages: 1-3(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.001
        摘要：Objective:To establish the quality control standard of Jiuweiqianghuo Concentrated Pills.Methods:Applying TLC to indentify Rhizoma atractylodis、Rhizoma Chuangxiong and Radix Scutellariae,Using HPLC to determine the content of baicalin of Radix Scutellariae.Results:Rhizoma atractylodis、Rhizoma Chuangxiong and Radix Scutellariae could be indentified by TLC;baicalin showed a good linear relationship at the rang of 0.0304～0.2128μg,r0.9999.The average recovery was 99.6%,and RSD was 3.7%.Conclusion:The methods are accurate and quick,and can be used for the quality control of Jiuweiqianghuo Concentrated Pills.  
        关键词：Jiuweiqianghuo Concentrated Pills;TLC;HPLC;baicalin   
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        发布时间：2024-09-23
      • Issue 5, Pages: 3-4(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.002
        摘要：目的 :用药效学方法筛选喘平方的提取工艺。方法 :观察不同提取工艺样品的镇咳和平喘作用。结果 :离子交换树脂法较酸水 乙醇提取法的平喘效果为好 ,单味提取与复方提取两种方法提取相差不大。结论 :离子树脂法是较佳的提取工艺。  
        关键词：喘平粉雾剂;酸水提醇沉法;离子交换树脂法   
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        发布时间：2024-09-23
      • NI Yan,HAO Xu-liang,LI Xian-rong
        Issue 5, Pages: 5-7(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.003
        摘要：Objective:To establish the quality standard for Xiongzhizhentong Capsules.Methods:Thin layer chromatography was used for simultaneous of Rhizoma chuanxiong,Radix Paeoniae Alba,Radix Bupleuri and Radix Ginseng.Ferulic acid was determined by HPLC.Results:The TLC sports developed were fairly clear,and the blank test showed no interference.The linearity for ferulic acid was obtained over the range of 0^1025～0.5125μg and the average recovery of ferulic acid was 98^8%.with RSD 1.59%.Conclusion:The method is reliable.The results are stable with good reproducible.It can be used for quality control of the capsules.  
        关键词：Xiongzhizhentong Capsules;quality standard;ferulic acid;HPLC   
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        发布时间：2024-09-23
      • QU Xian-you1,TIAN Hua-yong2,PENG Fang-sheng2
        Issue 5, Pages: 8-9(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.004
        摘要：Objective:To establish the quality standard of Guilong pill.Methods:Swertia davidi were indnetified by TLC.To determine the contents of Swertine in Guilong pill by HPLC.Results and Conclusions:The average recovery and RSD were 95^1% and 1^21%.The method was available for quality control of Guilong pill.  
        关键词：Guilong pill;Swertine;HPLC   
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        发布时间：2024-09-23
      • ZHU Zhao-jing1,DENG Kai-ying2
        Issue 5, Pages: 10-11(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.005
        摘要：Objective:To improve on the HPLC method for determination of Ginsenosid Rg1 and Ginsenoside Re in Hongshen （Red Ginsen）.Method:A C 18 column was used.The mobile phase consisted of CH-3CN-H-2O（20∶80） and the detection wavelength was 203nm.Results:The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0^6008～6^0080μg,0^4032～4.0320 μg;the average recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 98^5%（RSD1^3%,n6） and 99^2%（RSD1^1%,n6）.Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of Hongshen.  
        关键词：Hongshen;Ginsenoside Rg1;Ginsenoside Re;HPLC   
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        发布时间：2024-09-23
      • Issue 5, Pages: 12-13(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.006
        摘要：目的 :应用高效液相色谱法测定冠心康片中淫羊藿苷的含量。方法 :采用BDS C18柱 （5 μm ,4 6mm× 2 5 0mm） ;乙腈 水 四甲基氢氧化铵 （2 80∶70 2∶1,用磷酸调pH4 8）为流动相 ;检测波长 2 70nm。结果 :线性范围为 1 0 37～ 6 2 2 2 μg,r =0 9995 ,回收率为 97 15 % ,RSD =1 82 %。结论 :该方法用于测定冠心康片中淫羊藿苷的含量简便、可靠、准确 ,可用于该制剂的质量控制。  
        关键词：高效液相色谱法;淫羊藿苷;冠心康片   
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        发布时间：2024-09-23
      • Issue 5, Pages: 13-14(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.007
        摘要：目的 :建立交心安神丸中盐酸小檗碱的含量测定方法。方法 :测定波长λs=345nm ,λR=2 2 0nm ,展开剂为苯 醋酸乙酯 甲醇 异丙醇 浓氨 （6∶3∶1 5∶0 5 ）。结果 :盐酸小檗碱的平均回收率为 96 71% ,RSD =3 2 4 %。结论 :该方法简便、快速、准确 ,可用于交心安神丸的质量控制。  
        关键词：交心安神丸;盐酸小檗碱;薄层扫描法   
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        发布时间：2024-09-23
      • Vol. 0, Issue 5, Pages: 14(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.008
          
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        发布时间：2024-09-23
      • ZHANG Tao-tao,SU Xing-wen,Jiang Wei-jian,HUANG Yi-jun,QIU Peng-xin,YAN Guang-mei
        Issue 5, Pages: 15-18(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.009
        摘要：OBJECTIVE To study whether ZGP can prevent cerebellar granule neurons from apoptosis induced by glutamate and its possible molecular mechanisms. METHODS The neurons were stained with fluorescein diacetate to observe their survival rate or stained with Hoechst 33258 to analyze their nuclear morphology. Internucleosomal DNA fragmentation of neurons was analyzed with agarose gel electrophoresis. RESULTS ZGP could increase the survival rate of cultured CGNSs in the medium containing 300μmol·L -1 glutamate concentration-dependently, and could prevent the neuronal nuclei from shrinkage, condensation and cleavage and nuclear DNA fragmentation induced by glutamate. LY294002 suppressed the protective effect of ZGP, PD98059 was able to block the excitotoxin of glutamate. CONCLUTION ZGP protected rat CGNs from appoptosis induced by glutamate and this effect was postulated to dependent PI3-K/Akt signaling pathway.  
        关键词：Zuogui Pill;cerebellar granule neurons;apoptosis;molecular mechanisms   
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        发布时间：2024-09-23
      • LIU Ming-ping1,HUANG Zhao-sheng1,SUN Wei-guang2
        Issue 5, Pages: 19-21(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.010
        摘要：Objective To observe the effect of Yang Shou Dan（YSD） on IL-1 β、AchE response elements of APP protein. Methods:Alzheimer’s disease （AD） combining Yang deficiency of Kidney rats model was established by intraperitoneral injection of D-galgctose and intramuscularly injection of prednisolone acetate combined with ibotenic acid injection into bilateral nucleus basalis of Meynert. IL-1 β and AchE were inspected in the brain of rats. Results IL-1 β in model rat was significantly increased compared with the control group （P<0.01）. YSD and Piracetam tablet reduced IL-1 β notably （P<0.01,P<0.05） Compared with the model. However, there was no difference with control group （P>0.05）. AchE in hippocampus and cerebral cortex was elevated in model rat brain （P<0.01）. YSD lowered AchE and the function of high dose was prominence （P<0.01）. Conclusion:It is indicated that YSD can regulate IL-1 β, AchE response elements of APP protein in brain of mdel rat towards the normal levels.  
        关键词：Yangshou Dan;Alzheimer’s disease;Yang Deficiency of Kidney;Interleuking-1 beta;acetylcholinesterase   
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        发布时间：2024-09-23
      • WANG Ning,LIOU Qing-yun,PENG Dai-yin,DAI Min,TANG Jiou-yi,LI JING-Ling
        Issue 5, Pages: 22-24(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.011
        摘要：Model of repetitious cerebral ischemia reperfusion in mice induced by ligaturing biliateral carotid arteries was established to observe the protective effects and mechanisms of Tongqiao Huoxue Tang. The results show that Tongqiao Huoxue Tang can decrease the brain index, brain water content, enhance the activities cf SOD、 Na-K-ATP- ase 、Ca 2 -ATP- ase , and decrease the content of MDA.  
        关键词：Tongqiao Huoxue Tang;Repetitious cerebral ischemia reperfusion;mice;mechanisms   
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      • Issue 5, Pages: 24-25(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.012
        摘要：目的 :实验研究复方血竭胶囊对急性血瘀大鼠血液流变学的影响。方法 :采用皮下注射盐酸肾上腺素加冷刺激形成急性血瘀大鼠模型。结果 :复方血竭胶囊高、中、低剂量及复方丹参片能使急性“血瘀”大鼠全血粘度、血浆粘度、红细胞压积有明显的降低。结论 :复方血竭胶囊能改善急性血瘀大鼠的粘、浓、凝、聚等血液流变学异常 ,从而具有活血化瘀作用 ,有利于防止血栓性疾病。  
        关键词：血竭;血瘀;大鼠   
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        发布时间：2024-09-23
      • WANG Jian,JING Heng
        Issue 5, Pages: 26-29(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.013
        摘要：Objective: To study the mechanism of Naoluoxintong in preventing and treating on cerebral ischemia and reperfusion damage.Methods: The cerebral ischemia and reperfusion model with Qi-deficiency and blood stasis syndrome was established to investigate the preventive and therapeutical effect. The expressions of Bcl-2 protein and Bax protein were observed by the method of immunohistochemistry; the apoptosis was observed by the method of TUNEL Results:Naoluoxintong can counteract neural cell apoptosis after cerebral ischemia and reperfusion;Naoluoxintong can increase the expression of Bcl-2 protein while reduce the expression of Bax protein. Conclusions: Naoluoxintong can counteract neural cell apoptosis after cerebral ischemia and reperfusion with the effection on apoptosis-related protein.  
        关键词：Naoluoxintong;Cerebral ischemia and reperfusion damage;apoptosis   
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        发布时间：2024-09-23
      • HE Li-qun,WANG Yi,CAO He-xin,LI Jun
        Issue 5, Pages: 29-32(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.014
        摘要：Objective:To observe the effect of kangxianling decoction on PDGF-mRNA,TNF α-mRNA expression of CRF Rat renal tissue. Methods:The expression of PDGF-mRNA, TNF α-mRNA level of CRF Rats renal tissue were determined by RT-PCR and the blood TNF-α content of CRF Rats was measured by ELISA method in all groups two months later. Results:There are high expression of PDGF-mRNA, TNF α-mRNA in renal tissue of the model group and there are slightly expression in false operation group. The kangxianling decoction could suppress significantly the expression of PDGF-mRNA, TNF α-mRNA level of CRF Rats renal tissue. The blood TNF-α content of model group was higher than those of the normal group （P<0.01）. The kangxianling decoction could decrease significantly the blood TNF-α content of model group and its effect was better than those of Dan-shen root, Rhubarb and monopril groups （P<0.01）.Conclusion:The kangxianling decoction could relieve the ECM deposition in renal glomerulus and interstitium, improve kidney fibrosis and retard the deterioration of renal function by suppressing the expression of PDGF-mRNA, TNF α-mRNA level of CRF Rat renal tissue, decreasing the blood TNF-α content and the effect of the PDGF, TNF α on cell proliferation of renal tissue.  
        关键词：chronic renal failune;animal model;PDGF-mRNA;TNF α-mRNA   
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        发布时间：2024-09-23
      • Issue 5, Pages: 33-34(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.015
        摘要：通过小鼠抗炎、肠推进等试验 ,显示通便灵胶囊口服给药能明显缩短小鼠排黑便时间和增加排便数最 ,降低腹腔毛细血管通透性 ,对二甲苯所致的小鼠耳肿胀、肠推进无明显影响。结果表明 ,通便灵胶囊有明显的通便作用和一定的抗炎作用。  
        关键词：通便灵胶囊;抗炎;便泌   
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        发布时间：2024-09-23
      • LIN Cheng-ren1,MA Xue-ying1,WANG Min1,ZONG Hong-xin2,LIU Jian-xun1
        Issue 5, Pages: 35-38(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.016
        摘要：Objective To study the therapeutic effect of Qingxiang Pill （concentrated Pill） on Mammary Gland Hyperplasia（MGH）. Methods Injecting rabbits with estradiol benzoate induced the model of MGH, and rabbits in the normal control received saline injection only. Changes of breast diameter and length, mammary gland volume and weight were measured. The concentrations of sex hormones, SOD, and MDA in serum were estimated. The organ coefficients were measured, too. Pathologic changes in morphology due to MGH in rabbits were observed by microscopy. Results The medicine could improve hyperplasia of mammary glands in rabbits, reduce the enlargement value of breast diameter and length, mammary gland volume and weight, decrease the numbers of acinus and lobule of mammary glands. It could also decrease E-2 level in serum, and increase serum SOD activity and the coefficient of thymus. Conclusion Qingxiang Pill （concentrated Pill） can regulate sex hormone level, increase the ability to get rid of free radicals, and improve the pathologic changes in morphology due to MGH in rabbits.  
        关键词：Qingxiang pill;Rabbit;Mammary gland hyperplasia;Sex hormone;Free radicals   
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        发布时间：2024-09-23
      • REN Ying,GUO Jing,SONG Chong-shun,TIAN Jin-zhou,SHI Jing,ZHANG Man,YANG Jin-duo,YE Feng-hua
        Issue 5, Pages: 39-41(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.017
        摘要：By using model of MCAO which is made by additional minute line （Φ 3mm） in arteria carotis intema for 120min of anesthetic rat. The experimental result showed that JNN could significantly alleviaete these changes:JNN 11.25g medicinal herbs/kg and 5.625g medicinal herbs/kg could improve the decrease of whole blood viscosity and plasma viscosity （P<0.01, P<0.05 compared with vehicle group）; could inhibit of the erythrocyte aggregation （P<0.01, P<0.05 compared with vehicle group）; could increase of the erythrocyte deformational activity （P<0.05, compared with vehicle group）; could increase content of 6-keto-PGF-{2α} （JNN 11.25g 5.625 medicinal herbs/kg P<0.01, compared with vehicle group） and IL-1 β（JNN 11.25g medicinal herbs/kg P<0.05 compared with vehicle group）, IL-2（JNN 11.25g, 5.625 medicinal herbs/kg P<0.05 compared with vehicle group） in blood serum （JNL 11.25g medicinal herbs/kg P<0.05,P<0.05 compared with vehicle group）; Could increase of TXBS（JNN 11.25g, 5.625 medicinal herbs/kg P<0.01, compared with vehicle group） in blood plasma.  
        关键词：middle cerebral artery thrombosis occlusion;jiannaoning;blood rheologic observation   
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        发布时间：2024-09-23
      • Issue 5, Pages: 42-44(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.018
        摘要：目的 :研究舒络通胶囊的镇痛和活血作用及机理。方法 :观察舒络通胶囊的镇痛作用及对全血粘度、血小板聚集功能、脑血液循环、血管活性物质等的影响。结果 :舒络通胶囊能明显减轻小鼠热刺激性疼痛和机械性压痛 ;显著降低大鼠全血粘度 ,抑制血小板聚集 ;增加小鼠脑血流量 ;明显提高利血平化小鼠脑组织内 5 HT含量 ;拮抗NE引起的家兔主动脉的过度收缩。结论 :舒络通胶囊能治疗血管性头痛 ,可能与其作用与抑制血小板聚集 ,调节 5 HT等神经递质释放 ,改善脑血管舒缩功能等有关。  
        关键词：舒络通胶囊;血管性头痛;药效;神经递质   
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        发布时间：2024-09-23
      • Issue 5, Pages: 45-47(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.019
        摘要：仙竹降糖胶囊的药效学实验结果显示 :口服仙竹降糖胶囊可明显降低肾上腺素性高血糖大鼠的血糖水平 ,明显降低四氧嘧啶性糖尿病大鼠的血糖含量、MDA含量 ,提高SOD活力及胰岛素水平 ,降低甘油三酯和胆固醇含量。对正常小鼠的血糖、糖耐量未见明显的影响。提示仙竹降糖胶囊对高血糖大鼠具有保护胰岛B细胞、抗脂质过氧化及调节机体代谢的作用 ,并在此基础上从方药组成分析该方治疗糖尿病的作用机制。  
        关键词：仙竹降糖胶囊;药效学;糖尿病;作用机制   
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      • Issue 5, Pages: 48-50(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.020
        摘要：目的 :探讨葛根芩连微丸止泻止痢的药效作用。方法 :观察该药对小鼠小肠功能、小鼠腹泻、大鼠离体回肠运动机能以及体内外抗菌作用的影响。结果 :该药能够明显抑制正常小鼠小肠推进运动 ;对抗新斯的明引起的小鼠小肠推进机能亢进 ;抑制蓖麻油引起的小鼠腹泻 ;抑制大鼠离体回肠正常运动 ;对抗Ach引起的大鼠离体回肠痉挛性收缩 ;降低痢疾杆菌感染小鼠的死亡率 ;体外对痢疾杆菌等致病菌具有不同程度的抑制作用。结论 :葛根芩连微丸具有止泻止痢的作用。  
        关键词：葛根芩连微丸;肠功能;解痉;止泻;止痢   
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      • Issue 5, Pages: 51-52(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.021
        摘要：目的 :观察血压平对高血压血管重塑时血管紧张素Ⅱ （AngⅡ ）及醛固酮 （Ald）的影响。方法 :建立两肾一夹高血压大鼠 （2KlC RHR）模型 ,观测血压变化 ,放免法检测血浆AngⅡ及 2 4h尿Ald排泄量。结果 :高血压组与正常对照组比较 ,其AngⅡ及Ald水平明显升高 ;在高血压大鼠组中 ,血压平及罗布麻两组 ,治疗后AngⅡ、Ald及血压水平明显下降 ,与治疗前比较有显著性差异 ,与生理盐水组比较也有显著性差异。结论 :血压平对 2K1C RHR有明显、稳定的降压作用 ,对高血压血管重塑有一定的逆转作用。  
        关键词：肾血管性高血压大鼠;血管重塑;血压平胶囊   
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      • Issue 5, Pages: 53-54(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.022
        摘要：目的 :观察漏芦提取液对OLDL诱导U937细胞表面CD36表达的影响。方法 :先用OLDL与U937细胞孵育造成泡沫细胞模型 ,同时用漏芦提取液对泡沫细胞进行干预 ,采用流式细胞仪检测CD36的表达情况。结果 :漏芦提取液 1g L、5 0 0mg L、2 5 0mg L可不同程度地抑制CD36的表达 ,且三者呈剂量依赖关系。结论 :漏芦抗AS作用可能的机理与其抑制CD36的表达有关。  
        关键词：漏芦;泡沫细胞;CD36;OLDL;U937   
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        发布时间：2024-09-23
      • YAO Kui-wu,LI Jian-sheng,MA Li-jun,LI Su-yun,WU Ji-zhen,ZHANG Luo-xian
        Issue 5, Pages: 55-57(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.023
        摘要：Aim To study the nutritional status of the pneumonia patients in the aged and the effect of Dusuqing.Methods 19 normal young persons,20 normal aged persons and 30aged patients with pneumonia were enrolled to assess the nutritional status and the effect of Dusuqing.Results Anthropometrics measurements had an increase tendency after therpy in pneumonia groups. The contents of TRF,T 3,T 4,TSH,Co,IGF in aged group were lower than those in young group.The TRF,T 3,IGF-Ⅱlevel in pneumonia group were lower than those in normal aged group,but the Co and IL-β level in pneumonia group were high abviously compared with those in normal aged group. The TRF,T 3,IGF-Ⅱ level increased obviously,especially in Dusuqing group.The Co content decreased in both group after therapy,especially in Dusuqing group.The IL-Ⅰβ level decreased in Dusuqing group after therapy.Conclusion Malnutrition was one of the pathological characteristics of the aged pneumonia patients and Dusuqing could change this conditions.  
        关键词：pneumonia;aged;nutritional status;hormone;cytokine   
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        发布时间：2024-09-23
      • Issue 5, Pages: 57-58(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.024
        摘要：目的 :探讨通利胶囊治疗慢性前列腺炎的作用机制。方法 :6 0例患者分为 2组 ,治疗组口服通利胶囊 3颗 ,每日 2次 :对照组口服氧氟沙星 0 2g ,每日 2次。两组均以 4周为一疗程。观察两组治疗前、后的尿流动力学指标 ,前列腺按摩液的变化。结果 :治疗组治疗后的最大尿流率和平均尿流率与治疗前比较显著提高 ,最大后尿道压和最大尿道闭合压与治疗前明显下降 ,而对照组治疗前后改变不明显 ;治疗组治疗后的前列腺按摩液白细胞数与对照组比较显著减少。结论 :通利胶囊治疗慢性前列腺炎的作用机制可能是改善尿道压和尿道闭合压 ,缓解尿液反流。  
        关键词：通利胶囊;慢性前列腺炎;尿流动力学   
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        发布时间：2024-09-23
      • Issue 5, Pages: 59-61(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.025
        摘要：目的 :通过临床观察隆力福治疗冠心病的疗效。方法 :依照中药新药研究指导原则 ,将病例随机分为治疗组 （130例 ）和对照组 （12 9例 ） ,治疗组服用隆力福 ,对照组服用复方丹参片 ,皆以一个月为一疗程。结果 :治疗组总有效率高于对照组 ,有显著性差异 ,没有明显不良反应。  
        关键词：冠心病;心绞痛;隆力福   
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        发布时间：2024-09-23
      • Issue 5, Pages: 61-62(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.026
        摘要：应用消蛾片做抑菌实验 ,显示 0 1%的消蛾片溶液对溶血性链球菌、金黄色葡萄球菌、铜绿假单孢菌等八种常见致病菌有较明显的抑菌作用 ,抑菌环直径≥ 12mm。用消蛾片治疗扁桃体炎、咽炎、舌炎、口腔粘膜溃疡 15 1例 ,有效 14 6例 ,无效 5例 ,总有效率 96 6 %。随机设华素片对照治疗 131例 ,有效 10 7例 ,无效 2 2例 ,总有效率 81 6 % ,经 χ2 检验 ,P <0 0 1,消蛾片的疗效优于华素片对照组疗效。  
        关键词：口腔炎症;消蛾片;抑菌实验;疗效   
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        发布时间：2024-09-23
      • MA De-lian,GUO Ling,LI Xin-hua,LI Qian
        Issue 5, Pages: 63-65(2003) DOI: 10.13422/j.cnki.syfjx.2003.05.027
        摘要：The article reviews the immonological enhancement,anti-inflamation and immunosuppressive actions of Chinese medicinal herbs. The active compositions,formulae,mechanism of actions and new research progresses are summarized.It will provide sound basis for research and development of the category of herbs.  
        关键词：Chinese medicinal herbs;immunity;immunological enhancement;anti-inflamation and immunosuppressive actions;active composition   
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        发布时间：2024-09-23
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